Separate mechanisms regulating accumbal taurine levels during baseline conditions and following ethanol exposure in the rat

• Published in: Scientific reports 2024-10, Vol.14 (1), p.24166-13, Article 24166
• Main Authors: Ademar, Karin, Ulenius, Lisa, Loftén, Anna, Söderpalm, Bo, Adermark, Louise, Ericson, Mia
• Format: Article
• Language: English
• Subjects: 631/378/1697; 631/378/1788; 631/378/2586; 631/378/3920; 631/378/548; Action potential; Action Potentials - drug effects; Animals; Anion channels; Astrocytes; Astrocytes - drug effects; Astrocytes - metabolism; Calcium channels (L-type); Dopamine; Drug abuse; Drug delivery; Ethanol; Glutamic acid receptors (ionotropic); Humanities and Social Sciences; Male; Metabolic rate; Metabolism; Microdialysis; multidisciplinary; N-Methyl-D-aspartic acid receptors; Neurosciences; Neurovetenskaper; Nucleus accumbens; Nucleus Accumbens - drug effects; Nucleus Accumbens - metabolism; Rats; Rats, Wistar; Science; Science (multidisciplinary); Taurine; Taurine - metabolism; Taurine transporter
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-74449-7

Ethanol-induced dopamine release in the nucleus accumbens (nAc) is associated with reward and reinforcement, and for ethanol to elevate nAc dopamine levels, a simultaneous increase in endogenous taurine is required within the same brain region. By employing in vivo microdialysis in male Wistar rats combined with pharmacological, chemogenetic and metabolic approaches, our aim with this study was to identify mechanisms underlying ethanol-induced taurine release. Our results demonstrate that the taurine elevation, elicited by either systemic or local ethanol administration, occurs both in presence and absence of action potential firing or NMDA receptor blockade. Inhibition of volume regulated anion channels did not alter the ethanol-induced taurine levels, while inhibition of the taurine transporter occluded the ethanol-induced taurine increase, putatively due to a ceiling effect. Selective manipulation of nAc astrocytes using G q -coupled designer receptors exclusively activated by designer drugs (DREADDs) did not affect ethanol-induced taurine release. However, activation of G i -coupled DREADDs, or metabolic inhibition using fluorocitrate, rather enhanced than depressed taurine elevation. Finally, ethanol-induced taurine increase was fully blocked in rats pre-treated with the L-type Ca 2+ -channel blocker nicardipine, suggesting that the release is Ca 2+ dependent. In conclusion, while astrocytes appear to be important regulators of basal taurine levels in the nAc, they do not appear to be the main cells underlying ethanol-induced taurine release.