Biosynthesis of Co3O4 nanomedicine by using Mollugo oppositifolia L. aqueous leaf extract and its antimicrobial, mosquito larvicidal activities

• Published in: Scientific reports 2023-06, Vol.13 (1), p.9002-9002, Article 9002
• Main Authors: Gowthami, P., Kosiha, A., Meenakshi, S., Boopathy, G., Ramu, A. G., Choi, Dongjin
• Format: Article
• Language: English
• Subjects: 631/326; 631/45; 631/61; 631/92; Antifungal activity; Aquatic insects; Biosynthesis; Catalysis; Chemical reduction; Ciprofloxacin; Cosmetics; Crystals; Culex quinquefasciatus; E coli; Electron microscopes; Humanities and Social Sciences; Insecticides; Larvae; Larvicides; Leaves; Minimum inhibitory concentration; Mollugo; Mosquitoes; multidisciplinary; Nanoparticles; Nanotechnology; Permethrin; Plant extracts; Scanning electron microscopy; Science; Science (multidisciplinary); Spectroscopy; X-ray diffraction
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-35877-z

Nanotechnology is a relatively revolutionary area that generates day-to-day advancement. It makes a significant impact on our daily life. For example, in parasitology, catalysis and cosmetics, nanoparticles possess distinctive possessions that make it possible for them in a broad range of areas. We utilized Mollugo oppositifolia L. aqueous leaf extract assisted chemical reduction method to synthesize Co 3 O 4 nanoparticles. Biosynthesized Co 3 O 4 Nps were confirmed via UV–Vis spectroscopy, scanning electron microscope, X-ray diffraction, EDX, Fourier-transform infrared, and HR-TEM analysis. The crystallite size from XRD studies revealed around 22.7 nm. The biosynthesized Co 3 O 4 nanoparticle was further assessed for mosquito larvicidal activity against south-urban mosquito larvae Culex quinquefasciatus , and antimicrobial activities. The synthesized Co 3 O 4 particle ( 2 ) displayed significant larvicidal activity towards mosquito larvae Culex quinquefasciatus with the LD 50 value of 34.96 µg/mL than aqueous plant extract ( 1 ) and control Permethrin with the LD 50 value of 82.41 and 72.44 µg/mL. When compared to the standard antibacterial treatment, Ciprofloxacin, the Co 3 O 4 nanoparticle ( 2 ) produced demonstrates significantly enhanced antibacterial action against the pathogens E. coli and B. cereus . The MIC for Co 3 O 4 nanoparticles 2 against C. albicans was under 1 μg/mL, which was much lower than the MIC for the control drug, clotrimale, which was 2 µg per milliliter. Co 3 O 4 nanoparticles 2, with a MIC of 2 μg/mL, has much higher antifungal activity than clotrimale, whose MIC is 4 μg/mL, against M. audouinii .