Bacterial Infection and Hypersensitive Response Assays in Arabidopsis-Pseudomonas syringae Pathosystem

pathosystem has been used as an important model system for studying plant-microbe interactions, leading to many milestones and breakthroughs in the understanding of plant immune system and pathogenesis mechanisms. Bacterial infection and plant disease assessment are key experiments in the studies of...

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Bibliographic Details
Published in:Bio-protocol 2021-12, Vol.11 (24), p.e4268-e4268
Main Authors: Yuan, Minhang, Xin, Xiu-Fang
Format: Article
Language:English
Subjects:
Biology
Methods
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Summary:pathosystem has been used as an important model system for studying plant-microbe interactions, leading to many milestones and breakthroughs in the understanding of plant immune system and pathogenesis mechanisms. Bacterial infection and plant disease assessment are key experiments in the studies of plant-pathogen interactions. The hypersensitive response (HR), which is characterized by rapid cell death and tissue collapse after inoculation with a high dose of bacteria, is a hallmark response of plant effector-triggered immunity (ETI), one layer of plant immunity triggered by recognition of pathogen-derived effector proteins. Here, we present a detailed protocol for bacterial disease and hypersensitive response assays applicable to studies of interaction with various plant species such as s, , and tomato.
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.4268