L-Arginine protects ovine intestinal epithelial cells from lipopolysaccharide-induced intestinal barrier injury

This research aims to explore the effect of L-arginine (Arg) on ovine intestinal epithelial cells (IOECs) from lipopolysaccharide (LPS)-induced intestinal barrier injury. IOECs were incubated for 16 h and classified as 4 groups with the replacement of various mediums, including (1) control (Con), Ar...

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Bibliographic Details
Published in:Food and agricultural immunology 2019-01, Vol.30 (1), p.1067-1084
Main Authors: Zhang, Hao, Jin, Yaqian, Peng, Along, Guo, Shuang, Loor, Juan J., Wang, Hongrong
Format: Article
Language:English
Subjects:
Arginine
barrier functions
Epithelial cells
epithelium
immunology
Injuries
intestinal epithelial cells
Intestine
intestines
L-arginine
lipopolysaccharide
Lipopolysaccharides
Peptide transporter
peptide transporters
protein content
Protein transport
Proteins
Rapamycin
sheep
Supplements
TLR4 protein
Toll-like receptor 4
Toll-like receptors
TOR protein
Zonula occludens-1 protein
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Summary:This research aims to explore the effect of L-arginine (Arg) on ovine intestinal epithelial cells (IOECs) from lipopolysaccharide (LPS)-induced intestinal barrier injury. IOECs were incubated for 16 h and classified as 4 groups with the replacement of various mediums, including (1) control (Con), Arg-free Dulbecco's modified Eagle's F12 Ham medium (DMEM); (2) Arg treatment, Arg-free DMEM supplemented with 100 μM Arg; (3) LPS treatment, Arg-free DMEM supplemented with 10 μg/mL LPS; (4) LPS with Arg treatment, Arg-free DMEM supplemented with both 10 μg/mL LPS and 100 μM Arg. Arg supplementation to LPS-treated IOECs resulted in lower (P 
ISSN:0954-0105
1465-3443
1465-3443
DOI:10.1080/09540105.2019.1664417