Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)

Plant cells maintain a low luminal pH in the trans-Golgi-network/early endosome (TGN/EE), the organelle in which the secretory and endocytic pathways intersect. Impaired TGN/EE pH regulation translates into severe plant growth defects. The identity of the proton pump and proton/ion antiporters that...

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Bibliographic Details
Published in:eLife 2022-01, Vol.11
Main Authors: McKay, Daniel W, McFarlane, Heather E, Qu, Yue, Situmorang, Apriadi, Gilliham, Matthew, Wege, Stefanie
Format: Article
Language:English
Subjects:
Animals
Arabidopsis - genetics
Arabidopsis - physiology
Arabidopsis thaliana
Cation Transport Proteins - genetics
Cations - metabolism
Cell division
Chlorides
Chlorides - metabolism
Complementation
Drug therapy
Endocytosis
endomembrane
Endosomes
Endosomes - metabolism
Exocytosis
Flowers & plants
Gene Expression Regulation, Plant
Golgi apparatus
Homeostasis
Hydrogen-Ion Concentration
International economic relations
ion transport
KCC
Membrane proteins
NKCC
osmotic stress
Pathogens
pH effects
Photographic industry
Plant Biology
Plant cells
Potassium
Proteins
Protons
TGN
trans-Golgi Network - genetics
trans-Golgi Network - metabolism
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Summary:Plant cells maintain a low luminal pH in the trans-Golgi-network/early endosome (TGN/EE), the organelle in which the secretory and endocytic pathways intersect. Impaired TGN/EE pH regulation translates into severe plant growth defects. The identity of the proton pump and proton/ion antiporters that regulate TGN/EE pH have been determined, but an essential component required to complete the TGN/EE membrane transport circuit remains unidentified - a pathway for cation and anion efflux. Here, we have used complementation, genetically encoded fluorescent sensors, and pharmacological treatments to demonstrate that cation chloride cotransporter (CCC1) is this missing component necessary for regulating TGN/EE pH and function. Loss of CCC1 function leads to alterations in TGN/EE-mediated processes including endocytic trafficking, exocytosis, and response to abiotic stress, consistent with the multitude of phenotypic defects observed in knockout plants. This discovery places CCC1 as a central component of plant cellular function.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.70701