Recent advances in understanding the role of Cdk1 in the Spindle Assembly Checkpoint [version 1; peer review: 2 approved]

The goal of mitosis is to form two daughter cells each containing one copy of each mother cell chromosome, replicated in the previous S phase. To achieve this, sister chromatids held together back-to-back at their primary constriction, the centromere, have to interact with microtubules of the mitoti...

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Bibliographic Details
Published in:F1000 research 2020, Vol.9, p.57
Main Authors: Serpico, Angela Flavia, Grieco, Domenico
Format: Article
Language:English
Subjects:
Anaphase
Cell cycle
Cell division
Chromatids
Chromosomes
Cyclin B
Genomes
Incorporation
Kinases
Localization
Microtubules
Mitosis
Phosphatase
Phosphorylation
Proteins
Review
S phase
Sister chromatids
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Summary:The goal of mitosis is to form two daughter cells each containing one copy of each mother cell chromosome, replicated in the previous S phase. To achieve this, sister chromatids held together back-to-back at their primary constriction, the centromere, have to interact with microtubules of the mitotic spindle so that each chromatid takes connections with microtubules emanating from opposite spindle poles (we will refer to this condition as bipolar attachment). Only once all replicated chromosomes have reached bipolar attachments can sister chromatids lose cohesion with each other, at the onset of anaphase, and move toward opposite spindle poles, being segregated into what will soon become the daughter cell nucleus. Prevention of errors in chromosome segregation is granted by a safeguard mechanism called Spindle Assembly Checkpoint (SAC). Until all chromosomes are bipolarly oriented at the equator of the mitotic spindle, the SAC prevents loss of sister chromatid cohesion, thus anaphase onset, and maintains the mitotic state by inhibiting inactivation of the major M phase promoting kinase, the cyclin B-cdk1 complex (Cdk1). Here, we review recent mechanistic insights about the circuitry that links Cdk1 to the SAC to ensure correct achievement of the goal of mitosis.
ISSN:2046-1402
2046-1402
DOI:10.12688/f1000research.21185.1