Light regulates chlorophyll biosynthesis via ELIP1 during the storage of Chinese cabbage

Chlorophyll loss is a major problem during green vegetable storage. However, the molecular mechanism is still unclear. In this study, a 21 days of storage experiments showed chlorophyll content was higher in light-stored Chinese cabbage ( Brassica chinensis L . ) leaves than those in dark-stored sam...

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Bibliographic Details
Published in:Scientific reports 2022-06, Vol.12 (1), p.11098-11098, Article 11098
Main Authors: Wang, Tuoyi, Liu, Sijia, Tian, Shaonan, Ma, Tianyi, Wang, Wei
Format: Article
Language:English
Subjects:
631/449
631/449/1659
631/449/2661
631/449/2686
Biosynthesis
Brassica oleracea
Chlorophyll
Chromatin
Genomes
Humanities and Social Sciences
Immunoprecipitation
Localization
Methyltransferase
multidisciplinary
Pigments
Protoporphyrin
Protoporphyrin IX
Science
Science (multidisciplinary)
Shelf life
Transcriptomes
tRNA
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Summary:Chlorophyll loss is a major problem during green vegetable storage. However, the molecular mechanism is still unclear. In this study, a 21 days of storage experiments showed chlorophyll content was higher in light-stored Chinese cabbage ( Brassica chinensis L . ) leaves than those in dark-stored samples. Transcriptome analyses were performed on these samples to determine the effects of light. Among 311 differentially expressed genes (DEGs), early light-induced protein 1 (ELIP1) was identified as the main control gene for chlorophyll synthesis. Tissues and subcellular localization indicated that ELIP1 was localized in the nucleus. Motifs structure analyses, chromatin immunoprecipitation (ChIP) assays, luciferase reporter assays, and overexpression experiments demonstrated that ELIP1 regulated the expressions of genomes uncoupled 4 ( GUN4 ), Glutamyl-tRNA reductase family protein ( HEMA1 ), and Mg-protoporphyrin IX methyltransferase (CHLM) by binding to G-box-like motifs and affected chlorophyll biosynthesis during the storage of Chinese cabbage. It is a possible common tetrapyrrole biosynthetic pathway for chlorophylls, hemes, and bilin pigments in photosynthetic organisms. Our research also revealed that white light can be used as a regulatory factor to improve the storage ability and extent shelf life of Chinese cabbage.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-15451-9