Isolated Bacteria from the Uteri of Camels with Different Reproductive Backgrounds: A Study on Sampling Methodology, Prevalence, and Clinical Significance

• Published in: Veterinary sciences 2023-01, Vol.10 (1), p.39
• Main Authors: Asadi, Behnam, Seyedasgari, Fahimeh, Ashrafi Tamai, Iradj, Yarmohammadi, Mehdi, Ebadi, Reza, Kim, Ellen, Barin, Abbas
• Format: Article
• Language: English
• Subjects: Bacteria; Camelidae; Catheters; Cervix; Clinical significance; Embryo transfer; Experiments; Infections; Methods; microbiota; Microorganisms; pregnancy; Reproductive technologies; Sampling; Ultrasonic imaging; uterine discharge; Uterus; Vagina
• ISSN: 2306-7381; 2306-7381
• DOI: 10.3390/vetsci10010039

The objectives of this study were to comparatively identify the common bacterial isolates from the uteri of camels coming from different reproductive backgrounds after standardizing the sampling method and to investigate the association of clinically measurable parameters with uterine colonization by these isolates. The uterine samples from 856 dromedary camels yielded a total of 17 different bacterial species with a higher proportion of sub-fertile camel uteri being colonized by bacteria (66.6%) as compared to nulliparous, recently calved, and those with unknown reproductive history combined (44.2%; p < 0.05). Camels with body condition scoring < 3 and those with a consistently echogenic appearance of the uterine lumen by sonography were more likely to be positive on uterine culture, while the presence of pus in uterine discharge was not associated with the odds of bacterial isolation (p > 0.05). While certain strains were more likely to be obtained from the uteri of the sub-fertile group (p < 0.05), embryo transfer to camels with a positive uterine culture in the absence of other gross reproductive pathologies did not necessarily affect the overall pregnancy rate compared to recipients with a negative uterine culture (p > 0.05). In conclusion, a relatively high bacterial load can be identified from the uteri of both sub-fertile and normal dromedary camels, with a higher frequency among the former. The uterine ultrasonography and evaluation of the body condition score can help in identifying the camels in which uterus is contaminated by bacteria.