Revealing the influence of electron beam melted Ti-6Al-4V scaffolds on osteogenesis of human bone marrow-derived mesenchymal stromal cells

• Published in: Journal of materials science. Materials in medicine 2021-09, Vol.32 (9), p.97-97, Article 97
• Main Authors: Ødegaard, Kristin S., Ouyang, Lingzi, Ma, Qianli, Buene, Glenn, Wan, Di, Elverum, Christer W., Torgersen, Jan, Standal, Therese, Westhrin, Marita
• Format: Article
• Language: English
• Subjects: Alkaline phosphatase; Alkaline Phosphatase - metabolism; Alloys - chemistry; Biocompatibility; Biocompatible Materials - chemistry; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Bone and Bones - metabolism; Bone growth; Bone implants; Bone marrow; Bone Marrow - metabolism; Bone matrix; Bone Substitutes; Calcification, Physiologic; Calcium; Calcium - metabolism; Cbfa-1 protein; Cell Differentiation; Cell Survival; Cell viability; Ceramics; Chemistry and Materials Science; Collagen (type I); Collagen Type I, alpha 1 Chain - metabolism; Composites; Core Binding Factor Alpha 1 Subunit - metabolism; Differentiation (biology); Electron beams; Electrons; Glass; Humans; Long bone; Materials Science; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; Mesenchyme; Mineralization; Natural Materials; Osteoblastogenesis; Osteoblasts; Osteoblasts - metabolism; Osteogenesis; Osteogenesis - drug effects; Osteoid; Polymer Sciences; Porosity; Prostheses and Implants; Prosthesis Design; Regenerative Medicine/Tissue Engineering; Scaffolds; Stromal cells; Stromal Cells - metabolism; Surfaces and Interfaces; Surgical implants; Thin Films; Tissue Engineering Constructs and Cell Substrates; Tissue Scaffolds - chemistry; Titanium; Titanium - chemistry; Titanium base alloys; Transplants & implants
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-021-06572-0

Porous Titanium-6Aluminum-4Vanadium scaffolds made by electron beam-based additive manufacturing (AM) have emerged as state-of-the-art implant devices. However, there is still limited knowledge on how they influence the osteogenic differentiation of bone marrow-derived mesenchymal stromal cells (BMSCs). In this study, BMSCs are cultured on such porous scaffolds to determine how the scaffolds influence the osteogenic differentiation of the cells. The scaffolds are biocompatible, as revealed by the increasing cell viability. Cells are evenly distributed on the scaffolds after 3 days of culturing followed by an increase in bone matrix development after 21 days of culturing. qPCR analysis provides insight into the cells’ osteogenic differentiation, where RUNX2 expression indicate the onset of differentiation towards osteoblasts. The COL1A1 expression suggests that the differentiated osteoblasts can produce the osteoid. Alkaline phosphatase staining indicates an onset of mineralization at day 7 in OM. The even deposits of calcium at day 21 further supports a successful bone mineralization. This work shines light on the interplay between AM Ti64 scaffolds and bone growth, which may ultimately lead to a new way of creating long lasting bone implants with fast recovery times.