Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats

Introduction. Our previous study suggested that NSC-CM (neural stem cell-conditioned medium) inhibited cell apoptosis in vitro. In addition, many studies have shown that neurotrophic factors and microparticles secreted into a conditioned medium by NSCs had neuroprotective effects. Thus, we hypothesi...

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Published in:Stem cells international 2018-01, Vol.2018 (2018), p.1-7
Main Authors: Qu, Tingyu, Wang, Hao, Ma, Shuang, Li, Lan, Chen, Hui, Wang, Cuilan, Yang, HongNa, Fu, YaRu
Format: Article
Language:English
Subjects:
Animal models
Apoptosis
B-cell lymphoma
Bcl-2 protein
Brain-derived neurotrophic factor
Cerebral blood flow
Cerebral infarction
Conditioning
Epidermal growth factor
Injuries
Ischemia
Lymphocytes B
Lymphoma
Microparticles
Mitochondria
Neural stem cells
Neuroprotection
Neurotrophic factors
Occlusion
Rats
Reperfusion
Rodents
Stem cells
Stroke
Stroke (Disease)
Surgery
Ultrastructure
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Summary:Introduction. Our previous study suggested that NSC-CM (neural stem cell-conditioned medium) inhibited cell apoptosis in vitro. In addition, many studies have shown that neurotrophic factors and microparticles secreted into a conditioned medium by NSCs had neuroprotective effects. Thus, we hypothesized that NSC-CM had the capacity of protecting against cerebral I/R injury. Methods. Adult male Sprague-Dawley rats receiving middle cerebral artery occlusion surgery as an animal model of cerebral I/R injury were randomly assigned to two groups: the control group and NSC-CM-treated group. 1.5 ml NSC-CM or PBS (phosphate buffer saline) was administrated slowly by tail vein at 3 h, 24 h, and 48 h after ischemia onset. Results. NSC-CM significantly ameliorated neurological defects and reduced cerebral infarct volume, accompanied by preserved mitochondrial ultrastructure. In addition, we also found that NSC-CM significantly inhibited cell apoptosis in the ischemic hemisphere via improving the expression of Bcl-2 (B-cell lymphoma-2). Conclusion. NSC-CM might be an alternative and effective therapeutic intervention for ischemic stroke.
ISSN:1687-966X
1687-9678
1687-9678
DOI:10.1155/2018/4659159