Breeding of Indica glutinous cytoplasmic male sterile line WX209A via CRISPR/Cas9 mediated genomic editing

Glutinous cytoplasmic male sterile (CMS) line is necessary to select hybrid glutinous rice combination with high yield and quality. To develop glutinous CMS with low amylose content, in this study, we firstly knocked out the granule-bound starch synthase OsWaxy in 209B using CRISPR/Cas9 mediated gen...

Full description

Saved in:
Bibliographic Details
Published in:Czech Journal of Genetics and Plant Breeding 2019-01, Vol.55 (3), p.93-100
Main Authors: Wang, Xin, Han, Yue, Feng, Xuan, Li, Yun-Zhen, Qin, Bao-Xiang, Luo, Ji-Jing, Wei, Zheng, Qiu, Yong-Fu, Liu, Fang, Li, Rong-Bai
Format: Article
Language:English
Subjects:
Amylose
Breeding methods
CRISPR
Crop yield
Cytoplasmic male sterility
Genetic modification
glutinous rice
Granule-bound starch synthase
Hybridization
Males
oswaxy
Plant breeding
Rice
rice breeding
Starch synthase
Technology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Glutinous cytoplasmic male sterile (CMS) line is necessary to select hybrid glutinous rice combination with high yield and quality. To develop glutinous CMS with low amylose content, in this study, we firstly knocked out the granule-bound starch synthase OsWaxy in 209B using CRISPR/Cas9 mediated genome editing technology and successfully obtained a glutinous maintainer line WX209B. Comparing with maintainer line 209B, WX209B showed decreased amylose contents and similar agronomic characters. And then, through one generation of hybridization and two generations of backcrossing with WX209B as the male parent and 209A as the female parent, the glutinous CMS line WX209A was successfully achieved. Our study provides a strategy to efficiently breed for the glutinous cytoplasmic male sterile line by combining CRISPR/Cas9-mediated gene editing technology with conventional backcross breeding method in a short period, which prepares the ground for further breeding of hybrid glutinous rice variety.
ISSN:1212-1975
1805-9325
DOI:10.17221/197/2017-CJGPB