Ileal transposition in rats influenced glucose metabolism and HSP70 levels

• Published in: Open life sciences 2015-05, Vol.10 (1)
• Main Authors: Stygar, Dominika, Sawczyn, Tomasz, Skrzep-Poloczek, Bronisława, Karcz-Socha, Iwona, Doleżych, Bogdan, Zawisza-Raszka, Agnieszka, Augustyniak, Maria, Żwirska-Korczala, Krystyna, Karcz, Wojciech Konrad
• Format: Article
• Language: English
• Subjects: HSP70; ileal transposition; metabolic surgery; obesity surgery; type 2 diabetes mellitus
• ISSN: 2391-5412; 2391-5412
• DOI: 10.1515/biol-2015-0029

Objective: Ileal transposition procedure (IT), in combination with sleeve gastrectomy, is widely used to induce diabetes remission and to control related metabolic abnormalities. A transposition of a long segment of distal ileum in obese Zucker rats improved glucose tolerance 6 months after IT. The premise of our study was to to examine the long - term effects of ileum transposition on the liver glycolytic enzymes content in a euglycemic group of operated Zucker rats. Methods: Twenty male Zucker rats underwent either the transposition of 50% distal ileum or a sham surgery. Six months after surgery, liver tissue concentrations of glycogen synthase kinase alpha (GSK-3α), glucose 6-phosphatase (G6PC), glycogen phosphorylase (PYGM) and phosphofructokinase (PFK) and HSP70 were assessed by immunoenzymatic methods. Results: HSP70 values were significantly higher in the IT group compared to SHAM. G6PC liver concentrations in the IT group were almost 1.45-fold lower than in the SHAM operated rats. Statistical analyses (F-test) showed HSP70 levels were significantly related to caveolin-1and SHAM group. Conclusions: Lowered glycolytic enzyme concentrations assessed in the liver suggest positive effects on glucose metabolism in long-term observations.