Comprehensive Identification of the Pum Gene Family and Its Involvement in Kernel Development in Maize

• Published in: International journal of molecular sciences 2023-09, Vol.24 (18), p.14036
• Main Authors: Feng, Wenqi, Zhang, Hongwanjun, Cao, Yang, Yang, Cheng, Khalid, Muhammad Hayder Bin, Yang, Qingqing, Li, Wanchen, Wang, Yingge, Fu, Fengling, Yu, Haoqiang
• Format: Article
• Language: English
• Subjects: Abiotic stress; Amino acids; Chromosomes; gene expression; Genes; Genomes; kernel development; Localization; maize; MicroRNAs; Phylogenetics; Proteins; Pumilio RNA-binding proteins; Roles; Seeds
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms241814036

The Pumilio (Pum) RNA-binding protein family regulates post-transcription and plays crucial roles in stress response and growth. However, little is known about Pum in plants. In this study, a total of 19 ZmPum genes were identified and classified into two groups in maize. Although each ZmPum contains the conserved Pum domain, the ZmPum members show diversity in the gene and protein architectures, physicochemical properties, chromosomal location, collinearity, cis-elements, and expression patterns. The typical ZmPum proteins have eight α-helices repeats, except for ZmPum2, 3, 5, 7, and 14, which have fewer α-helices. Moreover, we examined the expression profiles of ZmPum genes and found their involvement in kernel development. Except for ZmPum2, ZmPum genes are expressed in maize embryos, endosperms, or whole seeds. Notably, ZmPum4, 7, and 13 exhibited dramatically high expression levels during seed development. The study not only contributes valuable information for further validating the functions of ZmPum genes but also provides insights for improvement and enhancing maize yield.