ATXN1 N-terminal region explains the binding differences of wild-type and expanded forms

• Published in: BMC medical genomics 2019-10, Vol.12 (1), p.145-145, Article 145
• Main Authors: Rocha, Sara, Vieira, Jorge, Vázquez, Noé, López-Fernández, Hugo, Fdez-Riverola, Florentino, Reboiro-Jato, Miguel, Sousa, André D, Vieira, Cristina P
• Format: Article
• Language: English
• Subjects: Amino Acid Motifs; Analysis; Animals; Ataxia; Ataxin-1 - chemistry; Ataxin-1 - genetics; Ataxin-1 - metabolism; Binding interface; Binding Sites; Expanded ATXN1; Genetic aspects; Humans; Molecular Docking Simulation; Neurodegenerative diseases; Peptides - metabolism; Protein Binding; Protein Interaction Domains and Motifs; Protein Structure, Tertiary; Protein-protein interaction; Protein-protein interactions; Risk factors; Spinocerebellar ataxias; Spinocerebellar Ataxias - genetics; Spinocerebellar Ataxias - pathology; Surface science; Wild-type ATXN1
• ISSN: 1755-8794; 1755-8794
• DOI: 10.1186/s12920-019-0594-4

Wild-type (wt) polyglutamine (polyQ) regions are implicated in stabilization of protein-protein interactions (PPI). Pathological polyQ expansion, such as that in human Ataxin-1 (ATXN1), that causes spinocerebellar ataxia type 1 (SCA1), results in abnormal PPI. For ATXN1 a larger number of interactors has been reported for the expanded (82Q) than the wt (29Q) protein. To understand how the expanded polyQ affects PPI, protein structures were predicted for wt and expanded ATXN1, as well as, for 71 ATXN1 interactors. Then, the binding surfaces of wt and expanded ATXN1 with the reported interactors were inferred. Our data supports that the polyQ expansion alters the ATXN1 conformation and that it enhances the strength of interaction with ATXN1 partners. For both ATXN1 variants, the number of residues at the predicted binding interface are greater after the polyQ, mainly due to the AXH domain. Moreover, the difference in the interaction strength of the ATXN1 variants was due to an increase in the number of interactions at the N-terminal region, before the polyQ, for the expanded form. There are three regions at the AXH domain that are essential for ATXN1 PPI. The N-terminal region is responsible for the strength of the PPI with the ATXN1 variants. How the predicted motifs in this region affect PPI is discussed, in the context of ATXN1 post-transcriptional modifications.