Effect of Byrsonima crassa and phenolic constituents on Helicobacter pylori-induced neutrophils oxidative burst

• Published in: International journal of molecular sciences 2012-01, Vol.13 (1), p.133-141
• Main Authors: Bonacorsi, Cibele, Raddi, Maria Stella G, da Fonseca, Luiz Marcos, Sannomiya, Miriam, Vilegas, Wagner
• Format: Article
• Language: English
• Subjects: antioxidant activity; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Byrsonima; Byrsonima crassa; chemiluminescence; Folk medicine; Gallic Acid - analogs & derivatives; Gallic Acid - chemistry; Gallic Acid - isolation & purification; Gallic Acid - pharmacology; Gastric Mucosa - cytology; Gastric Mucosa - microbiology; Gastric Mucosa - pathology; gastric ulcers; Helicobacter; Helicobacter pylori; Helicobacter pylori - drug effects; Helicobacter pylori - pathogenicity; Infections; Malpighiaceae; Malpighiaceae - chemistry; Malpighiaceae - metabolism; Methanol - chemistry; Neutrophils; Neutrophils - immunology; Neutrophils - microbiology; Oxidative stress; phenolic compounds; Phenols - chemistry; Phenols - isolation & purification; Phenols - pharmacology; Plant Extracts - chemistry; Plant Leaves - chemistry; Plant Leaves - metabolism; Reactive Oxygen Species - metabolism; Respiratory Burst - drug effects; Ulcers
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms13010133

Byrsonima crassa Niedenzu (Malpighiaceae) is used in Brazilian folk medicine for the treatment of diseases related mainly to gastric ulcers. In a previous study, our group described the gastric protective effect of the methanolic extract from the leaves of B. crassa. The present study was carried out to investigate the effects of methanolic extract and its phenolic compounds on the respiratory burst of neutrophils stimulated by H. pylori using a luminol-based chemiluminescence assay as well as their anti-H. pylori activity. The suppressive activity on oxidative burst of H. pylori-stimulated neutrophils was in the order of methyl gallate > (+)-catechin > methanol extract > quercetin 3-O-α-l-arabinopyranoside > quercetin 3-O-β-d-galactopyranoside > amentoflavone. Methyl gallate, compound that induced the highest suppressive activity with IC(50) value of 3.4 μg/mL, did not show anti-H. pylori activity. B. crassa could be considered as a potential source of natural antioxidant in gastric ulcers by attenuating the effects on the damage to gastric mucosa caused by neutrophil generated reactive oxygen species, even when H. pylori displays its evasion mechanisms.