Genome-Wide Identification and Transcriptional Analysis of the MYB Gene Family in Pearl Millet ( Pennisetum glaucum )

• Published in: International journal of molecular sciences 2023-01, Vol.24 (3), p.2484
• Main Authors: Lin, Miaohong, Dong, Zhuoyan, Zhou, Hongkai, Wu, Guanyu, Xu, Liang, Ying, Sheng, Chen, Miao
• Format: Article
• Language: English
• Subjects: Abiotic stress; Abscisic acid; Amino acids; Bioinformatics; Cereals; Chromosomes; Drought; Environmental effects; Evolution; Gene expression; Gene Expression Regulation, Plant; Genes; Genes, myb; Genomes; Localization; Millet; MYB gene; Nucleotide sequence; Nucleotides; Nutrients; Pattern analysis; pearl millet; Pennisetum - genetics; Pennisetum glaucum; PgMYB gene; Phylogenetics; Phylogeny; Positive selection; Protein sources; Proteins; Reverse transcription; Rice; Seeds; Stress, Physiological; Structural analysis; Subgroups; Synteny; transcriptional analysis
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24032484

The MYB gene family widely exists in the plant kingdom and participates in the regulation of plant development and stress response. Pearl millet ( R. Br.), as one of the most important cereals, is not only considered a good source of protein and nutrients but also has excellent tolerances to various abiotic stresses (e.g., salinity, water deficit, etc.). Although the genome sequence of pearl millet was recently published, bioinformatics and expression pattern analysis of the MYB gene family are limited. Here, we identified 208 PgMYB genes in the pearl millet genome and employed 193 high-confidence candidates for downstream analysis. Phylogenetic and structural analysis classified these PgMYBs into four subgroups. Eighteen pairs of segmental duplications of the PgMYB gene were found using synteny analysis. Collinear analysis revealed pearl millet had the closest evolutionary relationship with foxtail millet. Nucleotide substitution analysis (Ka/Ks) revealed PgMYB genes were under purifying positive selection pressure. Reverse transcription-quantitative PCR analysis of eleven R2R3-type PgMYB genes revealed they were preferentially expressed in shoots and seeds and actively responded to various environment stimuli. Current results provide insightful information regarding the molecular features of the MYB family in pearl millet to support further functional characterizations.