Colorimetric RT-LAMP Methods to Detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Standard diagnostic methods of Coronavirus Disease 2019 (COVID-19) rely on RT-qPCR technique which have limited point-of-care test (POCT) potential due to necessity of dedicated equipment and specialized personnel. LAMP, an isothermal nucleic acid amplification test (NAAT), is a promising technique...

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Bibliographic Details
Published in:Bio-protocol 2020-11, Vol.10 (21), p.e3804
Main Authors: Park, Gun-Soo, Baek, Seung-Hwa, Ku, Keunbon, Kim, Seong-Jun, Kim, Seung Il, Kim, Bum-Tae, Maeng, Jin-Soo
Format: Article
Language:English
Subjects:
Biology
Methods
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Summary:Standard diagnostic methods of Coronavirus Disease 2019 (COVID-19) rely on RT-qPCR technique which have limited point-of-care test (POCT) potential due to necessity of dedicated equipment and specialized personnel. LAMP, an isothermal nucleic acid amplification test (NAAT), is a promising technique that may substitute RT-qPCR for POCT of genomic materials. Here, we provide a protocol to perform reverse transcription LAMP targeting SARS-CoV-2. We adopted both real-time fluorescence detection and end-point colorimetric detection approaches. Our protocol would be useful for screening diagnosis of COVID-19 and be a baseline for development of improved POCT NAAT.
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.3804