Neutrophil Activation/Maturation Markers in Chronic Heart Failure with Reduced Ejection Fraction

Neutrophils are critically involved in the immune response. Inflammatory stimuli alter the expression status of their surface molecule toolset, while inflammation-stimulated granulopoiesis might also influence their maturation status. Data on neutrophil status in heart failure with reduced ejection...

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Bibliographic Details
Published in:Diagnostics (Basel) 2022-02, Vol.12 (2), p.444
Main Authors: Prausmüller, Suriya, Spinka, Georg, Stasek, Stefanie, Arfsten, Henrike, Bartko, Philipp Emanuel, Goliasch, Georg, Hülsmann, Martin, Pavo, Noemi
Format: Article
Language:English
Subjects:
Age
Body mass index
Cardiovascular disease
CD10
CD11b
CD64
CD66b
Chemokines
Coronaviruses
COVID-19
Ejection fraction
Enzymes
Flow cytometry
Heart failure
Inflammation
Laboratories
neutrophil activation
Neutrophils
Statistical analysis
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Summary:Neutrophils are critically involved in the immune response. Inflammatory stimuli alter the expression status of their surface molecule toolset, while inflammation-stimulated granulopoiesis might also influence their maturation status. Data on neutrophil status in heart failure with reduced ejection fraction (HFrEF) are scarce. The present study aims to evaluate the role of neutrophil CD11b, CD66b and CD64 expression in HFrEF. A total of 135 HFrEF patients and 43 controls were recruited. Mean fluorescence intensity of the activation/maturation markers CD11b, CD66b and CD64 was measured on neutrophils by flow cytometry. CD10 (neprilysin) expression was simultaneously determined. Neutrophil CD64 expression was higher in HFrEF compared with controls, while CD11b/CD66b levels were similar. Neutrophil CD11b and CD66b showed a significant direct correlation to neutrophil CD10 expression (rs = 0.573, < 0.001 and rs = 0.184, = 0.033). Neutrophil CD11b and CD66b correlated inversely with heart failure severity reflected by NT-proBNP and NYHA class (NT-proBNP: rs = -0.243, = 0.005 and rs = -0.250, = 0.004; NYHA class: = 0.032 and = 0.055), whereas no association for CD64 could be found. Outcome analysis did not reveal a significant association between the expression of CD11b, CD66b and CD64 and all-cause mortality ( = ns). The results underline the potential role of neutrophils in HFrEF disease pathophysiology and risk stratification and should stimulate further research, characterizing subpopulations of neutrophils and searching for key molecules involved in the downward spiral of inflammation and heart failure.
ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics12020444