The cytoskeleton of chondrocytes of Sepia officinalis (Mollusca, Cephalopoda): an immunocytochemical study

Our previous electron microscope study showed that chondrocytes from cephalopod cartilage possess a highly developed cytoskeleton and numerous cytoplasmic processes that ramify extensively through the tissue. We have now carried out a light microscope immunocytochemical study of chondrocytes from th...

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Bibliographic Details
Published in:European journal of histochemistry 2004-04, Vol.48 (2), p.159
Main Authors: Leone, F, Bairati, A, Vitellaro-Zuccarello, L
Format: Article
Language:English
Subjects:
Animals
Antibodies - analysis
Antibody Specificity
Cartilage - chemistry
Cartilage - drug effects
Cartilage - ultrastructure
Chickens
Chondrocytes - chemistry
Chondrocytes - drug effects
Chondrocytes - ultrastructure
Cytoskeleton - chemistry
Cytoskeleton - drug effects
Cytoskeleton - ultrastructure
Humans
Immunohistochemistry - methods
In Vitro Techniques
Mollusca - chemistry
Phalloidine - analogs & derivatives
Phalloidine - pharmacology
Rats
Rhodamines - pharmacology
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Summary:Our previous electron microscope study showed that chondrocytes from cephalopod cartilage possess a highly developed cytoskeleton and numerous cytoplasmic processes that ramify extensively through the tissue. We have now carried out a light microscope immunocytochemical study of chondrocytes from the orbital cartilage of Sepia officinalis to obtain indications as to the nature of the cytoskeletal components. We found clear positivity to antibodies against mammalian tubulin, vimentin, GFAP, and actin, but not keratin. The simultaneous presence of several cytoskeletal components is consistent with the hypothesis that cephalopod chondrocytes have the characteristics of both chondrocytes and osteocytes of vertebrates, which endow the tissue as a whole with some of the properties of vertebrate bone. We confirm, therefore, the presence in molluscs of the ubiquitous cytoskeletal proteins of metazoan cells that have remained highly conserved throughout phylogenetic evolution.
ISSN:1121-760X
2038-8306
DOI:10.4081/882