The cytoskeleton of chondrocytes of Sepia officinalis (Mollusca, Cephalopoda): an immunocytochemical study

Our previous electron microscope study showed that chondrocytes from cephalopod cartilage possess a highly developed cytoskeleton and numerous cytoplasmic processes that ramify extensively through the tissue. We have now carried out a light microscope immunocytochemical study of chondrocytes from th...

Full description

Saved in:
Bibliographic Details
Published in:European journal of histochemistry 2004-04, Vol.48 (2), p.159
Main Authors: Leone, F, Bairati, A, Vitellaro-Zuccarello, L
Format: Article
Language:English
Subjects:
Animals
Antibodies - analysis
Antibody Specificity
Cartilage - chemistry
Cartilage - drug effects
Cartilage - ultrastructure
Chickens
Chondrocytes - chemistry
Chondrocytes - drug effects
Chondrocytes - ultrastructure
Cytoskeleton - chemistry
Cytoskeleton - drug effects
Cytoskeleton - ultrastructure
Humans
Immunohistochemistry - methods
In Vitro Techniques
Mollusca - chemistry
Phalloidine - analogs & derivatives
Phalloidine - pharmacology
Rats
Rhodamines - pharmacology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c362t-d83f9730d9f017daeae38657d7e1d090da844ece71f3f153c6674f6ba4eacc313
cites
container_end_page
container_issue 2
container_start_page 159
container_title European journal of histochemistry
container_volume 48
creator Leone, F
Bairati, A
Vitellaro-Zuccarello, L
description Our previous electron microscope study showed that chondrocytes from cephalopod cartilage possess a highly developed cytoskeleton and numerous cytoplasmic processes that ramify extensively through the tissue. We have now carried out a light microscope immunocytochemical study of chondrocytes from the orbital cartilage of Sepia officinalis to obtain indications as to the nature of the cytoskeletal components. We found clear positivity to antibodies against mammalian tubulin, vimentin, GFAP, and actin, but not keratin. The simultaneous presence of several cytoskeletal components is consistent with the hypothesis that cephalopod chondrocytes have the characteristics of both chondrocytes and osteocytes of vertebrates, which endow the tissue as a whole with some of the properties of vertebrate bone. We confirm, therefore, the presence in molluscs of the ubiquitous cytoskeletal proteins of metazoan cells that have remained highly conserved throughout phylogenetic evolution.
doi_str_mv 10.4081/882
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_5899fd4728ff4860a93cc1cc4b897e44</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_5899fd4728ff4860a93cc1cc4b897e44</doaj_id><sourcerecordid>2397344091</sourcerecordid><originalsourceid>FETCH-LOGICAL-c362t-d83f9730d9f017daeae38657d7e1d090da844ece71f3f153c6674f6ba4eacc313</originalsourceid><addsrcrecordid>eNpFUU1v1DAUtBCIbhf-AorgAhIpduz4oze0AlqpqAeKxM16az-zXpI4tZPD_nuy7Iqe3pvRvHnSDCFrRq8E1eyT1s0zsmoo17XmVD4nK8YaVitJf12Qy1L2lEqzoJfkgrUN1VSLFdk_7LByhymVP9jhlIYqhcrt0uBzWmgsR_wDxwjLEqKLA3SxVO-_p66bi4OP1QbHHXRpTB4-XFcwVLHv5-F4nNwO--igq8o0-8Mr8iJAV_D1ea7Jz69fHjY39d39t9vN57vacdlMtdc8GMWpN4Ey5QEBuZat8gqZp4Z60EKgQ8UCD6zlTkolgtyCQHCOM74mtydfn2Bvxxx7yAebINp_RMq_LeQpug5tq40JXqhGhyC0pGC4c8w5sdVGoRCL19uT15jT44xlsvs05yWDYvUSpWoNV4vo3UnkciolY_j_lFF7rMYu1SyqN2eredujf9Kcu-B_AYeoiLE</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>876075937</pqid></control><display><type>article</type><title>The cytoskeleton of chondrocytes of Sepia officinalis (Mollusca, Cephalopoda): an immunocytochemical study</title><source>Publicly Available Content (ProQuest)</source><creator>Leone, F ; Bairati, A ; Vitellaro-Zuccarello, L</creator><creatorcontrib>Leone, F ; Bairati, A ; Vitellaro-Zuccarello, L</creatorcontrib><description>Our previous electron microscope study showed that chondrocytes from cephalopod cartilage possess a highly developed cytoskeleton and numerous cytoplasmic processes that ramify extensively through the tissue. We have now carried out a light microscope immunocytochemical study of chondrocytes from the orbital cartilage of Sepia officinalis to obtain indications as to the nature of the cytoskeletal components. We found clear positivity to antibodies against mammalian tubulin, vimentin, GFAP, and actin, but not keratin. The simultaneous presence of several cytoskeletal components is consistent with the hypothesis that cephalopod chondrocytes have the characteristics of both chondrocytes and osteocytes of vertebrates, which endow the tissue as a whole with some of the properties of vertebrate bone. We confirm, therefore, the presence in molluscs of the ubiquitous cytoskeletal proteins of metazoan cells that have remained highly conserved throughout phylogenetic evolution.</description><identifier>ISSN: 1121-760X</identifier><identifier>EISSN: 2038-8306</identifier><identifier>DOI: 10.4081/882</identifier><identifier>PMID: 15208084</identifier><language>eng</language><publisher>Italy: PAGEPress Publications</publisher><subject>Animals ; Antibodies - analysis ; Antibody Specificity ; Cartilage - chemistry ; Cartilage - drug effects ; Cartilage - ultrastructure ; Chickens ; Chondrocytes - chemistry ; Chondrocytes - drug effects ; Chondrocytes - ultrastructure ; Cytoskeleton - chemistry ; Cytoskeleton - drug effects ; Cytoskeleton - ultrastructure ; Humans ; Immunohistochemistry - methods ; In Vitro Techniques ; Mollusca - chemistry ; Phalloidine - analogs &amp; derivatives ; Phalloidine - pharmacology ; Rats ; Rhodamines - pharmacology</subject><ispartof>European journal of histochemistry, 2004-04, Vol.48 (2), p.159</ispartof><rights>Copyright PAGEPress Publications 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-d83f9730d9f017daeae38657d7e1d090da844ece71f3f153c6674f6ba4eacc313</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/876075937?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25752,27923,27924,37011,44589</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15208084$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leone, F</creatorcontrib><creatorcontrib>Bairati, A</creatorcontrib><creatorcontrib>Vitellaro-Zuccarello, L</creatorcontrib><title>The cytoskeleton of chondrocytes of Sepia officinalis (Mollusca, Cephalopoda): an immunocytochemical study</title><title>European journal of histochemistry</title><addtitle>Eur J Histochem</addtitle><description>Our previous electron microscope study showed that chondrocytes from cephalopod cartilage possess a highly developed cytoskeleton and numerous cytoplasmic processes that ramify extensively through the tissue. We have now carried out a light microscope immunocytochemical study of chondrocytes from the orbital cartilage of Sepia officinalis to obtain indications as to the nature of the cytoskeletal components. We found clear positivity to antibodies against mammalian tubulin, vimentin, GFAP, and actin, but not keratin. The simultaneous presence of several cytoskeletal components is consistent with the hypothesis that cephalopod chondrocytes have the characteristics of both chondrocytes and osteocytes of vertebrates, which endow the tissue as a whole with some of the properties of vertebrate bone. We confirm, therefore, the presence in molluscs of the ubiquitous cytoskeletal proteins of metazoan cells that have remained highly conserved throughout phylogenetic evolution.</description><subject>Animals</subject><subject>Antibodies - analysis</subject><subject>Antibody Specificity</subject><subject>Cartilage - chemistry</subject><subject>Cartilage - drug effects</subject><subject>Cartilage - ultrastructure</subject><subject>Chickens</subject><subject>Chondrocytes - chemistry</subject><subject>Chondrocytes - drug effects</subject><subject>Chondrocytes - ultrastructure</subject><subject>Cytoskeleton - chemistry</subject><subject>Cytoskeleton - drug effects</subject><subject>Cytoskeleton - ultrastructure</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>In Vitro Techniques</subject><subject>Mollusca - chemistry</subject><subject>Phalloidine - analogs &amp; derivatives</subject><subject>Phalloidine - pharmacology</subject><subject>Rats</subject><subject>Rhodamines - pharmacology</subject><issn>1121-760X</issn><issn>2038-8306</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpFUU1v1DAUtBCIbhf-AorgAhIpduz4oze0AlqpqAeKxM16az-zXpI4tZPD_nuy7Iqe3pvRvHnSDCFrRq8E1eyT1s0zsmoo17XmVD4nK8YaVitJf12Qy1L2lEqzoJfkgrUN1VSLFdk_7LByhymVP9jhlIYqhcrt0uBzWmgsR_wDxwjLEqKLA3SxVO-_p66bi4OP1QbHHXRpTB4-XFcwVLHv5-F4nNwO--igq8o0-8Mr8iJAV_D1ea7Jz69fHjY39d39t9vN57vacdlMtdc8GMWpN4Ey5QEBuZat8gqZp4Z60EKgQ8UCD6zlTkolgtyCQHCOM74mtydfn2Bvxxx7yAebINp_RMq_LeQpug5tq40JXqhGhyC0pGC4c8w5sdVGoRCL19uT15jT44xlsvs05yWDYvUSpWoNV4vo3UnkciolY_j_lFF7rMYu1SyqN2eredujf9Kcu-B_AYeoiLE</recordid><startdate>20040401</startdate><enddate>20040401</enddate><creator>Leone, F</creator><creator>Bairati, A</creator><creator>Vitellaro-Zuccarello, L</creator><general>PAGEPress Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>DOA</scope></search><sort><creationdate>20040401</creationdate><title>The cytoskeleton of chondrocytes of Sepia officinalis (Mollusca, Cephalopoda): an immunocytochemical study</title><author>Leone, F ; Bairati, A ; Vitellaro-Zuccarello, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-d83f9730d9f017daeae38657d7e1d090da844ece71f3f153c6674f6ba4eacc313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antibodies - analysis</topic><topic>Antibody Specificity</topic><topic>Cartilage - chemistry</topic><topic>Cartilage - drug effects</topic><topic>Cartilage - ultrastructure</topic><topic>Chickens</topic><topic>Chondrocytes - chemistry</topic><topic>Chondrocytes - drug effects</topic><topic>Chondrocytes - ultrastructure</topic><topic>Cytoskeleton - chemistry</topic><topic>Cytoskeleton - drug effects</topic><topic>Cytoskeleton - ultrastructure</topic><topic>Humans</topic><topic>Immunohistochemistry - methods</topic><topic>In Vitro Techniques</topic><topic>Mollusca - chemistry</topic><topic>Phalloidine - analogs &amp; derivatives</topic><topic>Phalloidine - pharmacology</topic><topic>Rats</topic><topic>Rhodamines - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leone, F</creatorcontrib><creatorcontrib>Bairati, A</creatorcontrib><creatorcontrib>Vitellaro-Zuccarello, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest - Health &amp; Medical Complete保健、医学与药学数据库</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>European journal of histochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leone, F</au><au>Bairati, A</au><au>Vitellaro-Zuccarello, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The cytoskeleton of chondrocytes of Sepia officinalis (Mollusca, Cephalopoda): an immunocytochemical study</atitle><jtitle>European journal of histochemistry</jtitle><addtitle>Eur J Histochem</addtitle><date>2004-04-01</date><risdate>2004</risdate><volume>48</volume><issue>2</issue><spage>159</spage><pages>159-</pages><issn>1121-760X</issn><eissn>2038-8306</eissn><abstract>Our previous electron microscope study showed that chondrocytes from cephalopod cartilage possess a highly developed cytoskeleton and numerous cytoplasmic processes that ramify extensively through the tissue. We have now carried out a light microscope immunocytochemical study of chondrocytes from the orbital cartilage of Sepia officinalis to obtain indications as to the nature of the cytoskeletal components. We found clear positivity to antibodies against mammalian tubulin, vimentin, GFAP, and actin, but not keratin. The simultaneous presence of several cytoskeletal components is consistent with the hypothesis that cephalopod chondrocytes have the characteristics of both chondrocytes and osteocytes of vertebrates, which endow the tissue as a whole with some of the properties of vertebrate bone. We confirm, therefore, the presence in molluscs of the ubiquitous cytoskeletal proteins of metazoan cells that have remained highly conserved throughout phylogenetic evolution.</abstract><cop>Italy</cop><pub>PAGEPress Publications</pub><pmid>15208084</pmid><doi>10.4081/882</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1121-760X
ispartof European journal of histochemistry, 2004-04, Vol.48 (2), p.159
issn 1121-760X
2038-8306
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_5899fd4728ff4860a93cc1cc4b897e44
source Publicly Available Content (ProQuest)
subjects Animals
Antibodies - analysis
Antibody Specificity
Cartilage - chemistry
Cartilage - drug effects
Cartilage - ultrastructure
Chickens
Chondrocytes - chemistry
Chondrocytes - drug effects
Chondrocytes - ultrastructure
Cytoskeleton - chemistry
Cytoskeleton - drug effects
Cytoskeleton - ultrastructure
Humans
Immunohistochemistry - methods
In Vitro Techniques
Mollusca - chemistry
Phalloidine - analogs & derivatives
Phalloidine - pharmacology
Rats
Rhodamines - pharmacology
title The cytoskeleton of chondrocytes of Sepia officinalis (Mollusca, Cephalopoda): an immunocytochemical study
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T06%3A29%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20cytoskeleton%20of%20chondrocytes%20of%20Sepia%20officinalis%20(Mollusca,%20Cephalopoda):%20an%20immunocytochemical%20study&rft.jtitle=European%20journal%20of%20histochemistry&rft.au=Leone,%20F&rft.date=2004-04-01&rft.volume=48&rft.issue=2&rft.spage=159&rft.pages=159-&rft.issn=1121-760X&rft.eissn=2038-8306&rft_id=info:doi/10.4081/882&rft_dat=%3Cproquest_doaj_%3E2397344091%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c362t-d83f9730d9f017daeae38657d7e1d090da844ece71f3f153c6674f6ba4eacc313%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=876075937&rft_id=info:pmid/15208084&rfr_iscdi=true