New insights into detecting alizarin from autofluorescence in marked glass eels

Alizarin detection in fish fins is extensively employed because it is easy to use. However, in eels, the eelGFP fluorescent protein may impede the detection of the fluorescent markers in the eel tissues. The study tests the effectiveness of three of the most up-to-date alizarin-detecting technologie...

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Bibliographic Details
Published in:Scientific reports 2022-09, Vol.12 (1), p.15985-15985, Article 15985
Main Authors: Gaillard, Mélanie, Parlanti, Edith, Sourzac, Mahaut, Couillaud, Franck, Genevois, Coralie, Boutry, Sébastien, Rigaud, Christian, Daverat, Françoise
Format: Article
Language:English
Subjects:
631/158
631/1647/245
631/1647/328
631/1647/527
704/829/826
Chemical Sciences
Endangered & extinct species
Endangered species
Environmental Sciences
Experimental design
Fins
Fluorescence
Fluorescence in situ hybridization
Fluorescent indicators
Humanities and Social Sciences
multidisciplinary
Science
Science (multidisciplinary)
Spectrum analysis
Wavelengths
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Summary:Alizarin detection in fish fins is extensively employed because it is easy to use. However, in eels, the eelGFP fluorescent protein may impede the detection of the fluorescent markers in the eel tissues. The study tests the effectiveness of three of the most up-to-date alizarin-detecting technologies on the living body and fins of European glass eels ( Anguilla anguilla L.). The findings demonstrated that the control group had a high autofluorescence at alizarin and eelGFP maxima bands. With fluorescence reflectance imaging (FRI), the eel living body autofluorescence impeded the detection of the marked eels. In contrast with experimental excitation-emission-matrix (EEM) fluorescence analyses, 99% of the marked eels were correctly assigned to their group from fluorescence analyses of their fin cellular contents. With epifluorometry (EPI), 100% of the marked eels were detected with the caudal fin tips when excited at 450–490 nm wavelengths due to a weaker autofluorescence signal. EEM and FRI assays unveiled an average fluorescence quenching 60% and 44% of the marked group respectively, in the alizarin and eelGFP maxima bands. The fluorescence quenching observed is discussed. Results will benefit experimental design by examining autofluorescence effects on mark detection and the development of non-invasive detection methods in this critically endangered species.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-18440-0