Highly specific fiber optic immunosensor coupled with immunomagnetic separation for detection of low levels of Listeria monocytogenes and L. ivanovii

Immunomagnetic separation (IMS) and immunoassays are widely used for pathogen detection. However, novel technology platforms with highly selective antibodies are essential to improve detection sensitivity, specificity and performance. In this study, monoclonal antibodies (MAbs) against Internalin A...

Full description

Saved in:
Bibliographic Details
Published in:BMC microbiology 2012-11, Vol.12 (1), p.275-275, Article 275
Main Authors: Mendonça, Marcelo, Conrad, Neida L, Conceição, Fabricio R, Moreira, Angela N, da Silva, Wladimir P, Aleixo, José Ag, Bhunia, Arun K
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - isolation & purification
Antibodies, Monoclonal - isolation & purification
Antigens, Bacterial - immunology
Bacteria
Bacteriological Techniques - methods
Biosensor
Cell adhesion & migration
Colony-forming cells
Detection
Detectors
Diagnosis
Equipment and supplies
Experiments
Fatalities
Female
Fiber optic sensor
Fiber Optic Technology - methods
Fiber optics
Fibers
Food
Food contamination & poisoning
Genetic aspects
Immunoassay - methods
Immunoassays
Immunoglobulin G
Immunoglobulin M
Immunomagnetic separation
Immunomagnetic Separation - methods
immunosensors
internalin
Internalin A
Light scattering sensor
Listeria
Listeria - isolation & purification
Listeria monocytogenes
Listeriosis
Media (selective)
Methodology
Mice
Mice, Inbred BALB C
Microbiology
Monoclonal antibodies
Monoclonal antibody
Optics
Pathogens
Physiological aspects
qPCR
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Immunomagnetic separation (IMS) and immunoassays are widely used for pathogen detection. However, novel technology platforms with highly selective antibodies are essential to improve detection sensitivity, specificity and performance. In this study, monoclonal antibodies (MAbs) against Internalin A (InlA) and p30 were generated and used on paramagnetic beads of varying diameters for concentration, as well as on fiber-optic sensor for detection. Anti-InlA MAb-2D12 (IgG2a subclass) was specific for Listeria monocytogenes and L. ivanovii, and p30-specific MAb-3F8 (IgM) was specific for the genus Listeria. At all bacterial concentrations (10³-10⁸ CFU/mL) tested in the IMS assay; the 1-μm diameter MyOne beads had significantly higher capture efficiency (P 
ISSN:1471-2180
1471-2180
DOI:10.1186/1471-2180-12-275