Highly Efficient Enzymatic Preparation of Daidzein in Deep Eutectic Solvents

Daidzein, which is scarce in nature, has gained significant attention due to its superior biological activity and bioavailability compared with daidzin. So far, it has been widely used in the medicine and health care products industries. The enzymatic approach for the preparation of daidzein has pre...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2017-01, Vol.22 (1), p.186-186
Main Authors: Cheng, Qi-Bin, Zhang, Li-Wei
Format: Article
Language:English
Subjects:
Bioavailability
Bioconversion
Biological activity
Daidzein
deep eutectic solvents
Enzymatic activity
enzymatic hydrolysis
Enzyme activity
Enzymes
Eutectic reactions
Glycine max - chemistry
Glycosides - chemistry
Health care
Hydrolysis
Intestinal Absorption - physiology
Isoflavones - chemistry
optimization
Process parameters
Reagents
Response surface methodology
Solvents
Stability analysis
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Summary:Daidzein, which is scarce in nature, has gained significant attention due to its superior biological activity and bioavailability compared with daidzin. So far, it has been widely used in the medicine and health care products industries. The enzymatic approach for the preparation of daidzein has prevailed, benefitted by its high efficiency and eco-friendly nature. Our present research aimed at providing a preparation method of daidzein by enzymatic hydrolysis of daidzin in a new "green" reaction medium-deep eutectic solvents (DESs). Herein, the DESs were screened via evaluating enzyme activity, enzyme stability and the substrate solubility, and the DES (ChCl/EG 2:1, 30 vol %) was believed to be the most appropriate co-solvent to improve the bioconversion efficiency. Based on the yield of daidzein, response surface methodology (RSM) was employed to model and optimize the reaction parameters. Under these optimum process conditions, the maximum yield of 97.53% was achieved and the purity of daidzein crude product reached more than 70%, which is more efficient than conversions in DESs-free buffer. Importantly, it has been shown that DESs medium could be reused for six batches of the process with a final conversion of above 50%. The results indicated that this procedure could be considered a mild, environmentally friendly, highly efficient approach to the economical production of daidzein, with a simple operation process and without any harmful reagents being involved.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules22010186