Establishment and Long-Term Expansion of Small Cell Lung Cancer Patient-Derived Tumor Organoids

Differential chemo-sensitivity of cancer cells, which is attributed to the cellular heterogeneity and phenotypic variation of cancer cells, is considered to be the main reason for tumor recurrence after chemotherapy. Here, we generated small cell lung cancer patient-derived tumor organoids and subje...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-01, Vol.22 (3), p.1349
Main Authors: Choi, Seon Young, Cho, Yong-Hee, Kim, Da-Som, Ji, Wonjun, Choi, Chang-Min, Lee, Jae Cheol, Rho, Jin Kyung, Jeong, Gi Seok
Format: Article
Language:English
Subjects:
patient avatar model systems
patient-derived tumor organoids
small cell lung cancer
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Summary:Differential chemo-sensitivity of cancer cells, which is attributed to the cellular heterogeneity and phenotypic variation of cancer cells, is considered to be the main reason for tumor recurrence after chemotherapy. Here, we generated small cell lung cancer patient-derived tumor organoids and subjected them to long-term expansion with the addition of WNT3A or R-spondin1. We confirmed that the organoids have similar genetic profiles, molecular characteristics, and morphological architectures to the corresponding patient tumor tissue during and after long-term expansion. Interestingly, the cellular heterogeneity of organoids is reflected in their differential response to cisplatin or etoposide. We propose to utilize the organoids as small cell lung cancer patient avatar models that would be ideal for investigating the mechanisms underlying tumor recurrence after chemotherapy, and would ultimately help to develop personalized medicine.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22031349