Roles of Three FgPel Genes in the Development and Pathogenicity Regulation of Fusarium graminearum

Fusarium head blight (FHB) is a devastating fungal disease caused by . Pectin lyase, a pectinase, acts on the α-1,4-glycosidic linkage of galacturonic acid primarily by β-elimination. In this study, three pectin lyase genes ( , , ) in were selected, and deletion mutants (Δ , , ) were constructed by...

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Published in:Journal of fungi (Basel) 2024-09, Vol.10 (10), p.666
Main Authors: Cai, Lu, Xu, Xiao, Dong, Ye, Jin, Yingying, Rashad, Younes M, Ma, Dongfang, Gu, Aiguo
Format: Article
Language:English
Subjects:
Biotechnology
Cell death
Cellulose
Deletion mutant
Enzymes
Experiments
Fungi
Fungicides
Fusarium graminearum
Fusarium head blight
Gene deletion
gene knockout
Gene regulation
Genes
Germination
Growth rate
Homologous recombination
Maximum likelihood method
Morphology
Mutants
Pathogenicity
Pathogens
Pectin
Pectin lyase
Pesticides
pH effects
Phylogenetics
Plant resistance
Proteins
Toxins
Virulence
Wheat
Yeast
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Summary:Fusarium head blight (FHB) is a devastating fungal disease caused by . Pectin lyase, a pectinase, acts on the α-1,4-glycosidic linkage of galacturonic acid primarily by β-elimination. In this study, three pectin lyase genes ( , , ) in were selected, and deletion mutants (Δ , , ) were constructed by homologous recombination for functional characterization. The gene deletions affected the morphology and growth rate of on pectin medium at various concentrations, with the growth rate of Δ being more significant. The growth of Δ colonies slowed at pH 4, with optimal growth at pH 6.5, whereas Δ and Δ exhibited greater inhibition at pH 8. Colony morphology and diameter of the deletion mutants showed no significant differences compared to the wild-type strain PH-1, and there was no effect on conidial production or germination rate. Pathogenicity assays demonstrated that gene deletion significantly reduced the ability of to infest corn silks and wheat ears, and that Δ showed a more pronounced reduction in pathogenicity on wheat spikes. In summary, the pectin lyase genes ( , , ) are involved in pectin utilization and are influenced by external pH conditions, which attenuate the pathogenicity of without affecting its vegetative growth or asexual spore formation. These findings elucidate the roles of these genes and provide a basis for controlling FHB.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof10100666