Characterization of sexual maturity-associated N6-methyladenosine in boar testes

• Published in: BMC genomics 2024-05, Vol.25 (1), p.447-447, Article 447
• Main Authors: Zhang, Pengfei, Zhang, Fei, Sui, Heming, Yang, Xingyu, Ji, Yiming, Zheng, Shenghao, Li, Wei, Cheng, Kun, Wang, Chonglong, Jiao, Jun, Zhang, Xiaodong, Cao, Zubing, Zhang, Yunhai
• Format: Article
• Language: English
• Subjects: Adenosine - analogs & derivatives; Adenosine - metabolism; Adults; Animals; Biological activity; Cell cycle; DNA methylation; Epigenetics; Fertility; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene set enrichment analysis; Genes; Infertility; Kinases; Livestock; m6A; Male; Metabolism; Methylation; MicroRNAs; mRNA; N6-methyladenosine; Phosphorylation; Pig; Proteins; RNA modification; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sexual Maturation - genetics; Sexual maturity; Signal Transduction; Sperm; Spermatogenesis; Swine; Swine - genetics; Testes; Testicular development; Testis - growth & development; Testis - metabolism
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-024-10343-w

The health and size of the testes are crucial for boar fertility. Testicular development is tightly regulated by epigenetics. N6-methyladenosine (m6A) modification is a prevalent internal modification on mRNA and plays an important role in development. The mRNA m6A methylation in boar testicular development still needs to be investigated. Using the MeRIP-seq technique, we identify and profile m6A modification in boar testes between piglets and adults. The results showed 7783 distinct m6A peaks in piglets and 6590 distinct m6A peaks in adults, with 2,471 peaks shared between the two groups. Enrichment of GO and KEGG analysis reveal dynamic m6A methylation in various biological processes and signalling pathways. Meanwhile, we conjointly analyzed differentially methylated and expressed genes in boar testes before and after sexual maturity, and reproductive related genes (TLE4, TSSK3, TSSK6, C11ORF94, PATZ1, PHLPP1 and PAQR7) were identified. Functional enrichment analysis showed that differential genes are associated with important biological functions, including regulation of growth and development, regulation of metabolic processes and protein catabolic processes. The results demonstrate that m6A methylation, differential expression and the related signalling pathways are crucial for boar testicular development. These results suggest a role for m6A modification in boar testicular development and provided a resource for future studies on m6A function in boar testicular development.