Lipophorin of the larval honeybee, Apis mellifera L

Most insects have a major lipoprotein species in the blood (hemolymph) that serves to transport fat from the midgut to the storage depots in fat body cells and from the fat body to peripheral tissues. The generic name lipophorin is used for this lipoprotein. In larvae of the honeybee, Apis mellifera...

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Bibliographic Details
Published in:Journal of lipid research 1985-02, Vol.26 (2), p.241-247
Main Authors: Robbs, S.L, Ryan, R.O, Schmidt, J.O, Keim, P.S, Law, J.H
Format: Article
Language:English
Subjects:
Amino Acids - analysis
Animals
Apidae
APIS MELLIFERA
Apolipoproteins - isolation & purification
Bees - analysis
BODY FLUIDS
Carrier Proteins - immunology
Carrier Proteins - isolation & purification
Larva - analysis
LARVAE
LARVAS
LARVE
Lipids - analysis
LIPOPROTEINAS
LIPOPROTEINE
LIPOPROTEINS
LIQUIDE BIOLOGIQUE
LIQUIDOS CORPORALES
Molecular Weight
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Summary:Most insects have a major lipoprotein species in the blood (hemolymph) that serves to transport fat from the midgut to the storage depots in fat body cells and from the fat body to peripheral tissues. The generic name lipophorin is used for this lipoprotein. In larvae of the honeybee, Apis mellifera, a lipophorin has been found with properties that correlate well with those of the only other lipophorin reported for an immature insect, that of the tobacco hornworm, Manduca sexta. The honeybee lipophorin (Mr = 530,000) has a density of 1.13 g/ml, contains approximately 41% lipid and 59% protein, and contains two apoproteins, apoLp-I, Mr = 250,000 and apoLp-II, Mr = 80,000, both of which are glycosylated. The lipids consist predominantly of polar lipids, of which phospholipids and diacylglycerols represent 60% of the total. When the intact lipophorin is treated with trypsin, apoLp-I is rapidly proteolyzed, while apoLp-II is resistant, indicating a difference in exposure of the two apoproteins to the aqueous environment. Honeybee apoLp-II cross-reacts with antibodies to M. sexta apoLp-II, but not to anti-M. sexta apoLp-I. No cross-reactivity of honeybee apoLp-I to anti-M. sexta apoLp-I was observed.
ISSN:0022-2275
1539-7262
DOI:10.1016/S0022-2275(20)34394-7