Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice

Telomeres cap the ends of eukaryotic chromosomes to maintain genomic stability and integrity during an organism’s lifespan. The length of telomeres inevitably shortens due to DNA replication, genotoxic agents, and biological aging. A limited number of cell types, e.g., stem cells, germline cells, an...

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Published in:Scientific reports 2021-07, Vol.11 (1), p.15569-15569, Article 15569
Main Authors: Kosebent, Esra Gozde, Ozturk, Saffet
Format: Article
Language:English
Subjects:
631/80
631/80/509
Aging
Chromosomes
DNA biosynthesis
Embryos
Fertility
Follicles
Gene expression
Genotoxicity
Humanities and Social Sciences
Infertility
Life span
Molecular modelling
multidisciplinary
Ovaries
Proteins
RNA-directed DNA polymerase
Science
Science (multidisciplinary)
Stem cell transplantation
Stem cells
Telomerase
Telomerase reverse transcriptase
Telomere-binding protein
Telomeres
TRF2 protein
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description Telomeres cap the ends of eukaryotic chromosomes to maintain genomic stability and integrity during an organism’s lifespan. The length of telomeres inevitably shortens due to DNA replication, genotoxic agents, and biological aging. A limited number of cell types, e.g., stem cells, germline cells, and early embryos can elongate shortened telomeres via the enzymatic action of telomerase, which is composed of telomerase reverse transcriptase (TERT) and telomerase RNA component ( Terc ). Additionally, telomere-associated proteins including telomeric repeat binding factor 1 (TRF1) and 2 (TRF2), as well as protection of telomeres 1a (POT1a), bind to telomeres to maintain their structural integrity and length. During ovarian aging in mammals, telomeres progressively shorten, accompanied by fertility loss; however, the molecular mechanism underlying this attrition during follicle development remains unclear. In this study, the primary, secondary, preantral, and antral follicles were obtained either from 6-week-old adult (n = 19) or 52-week-old aged (n = 12) mice. We revealed that the Tert , Terc , Trf1 , Trf2 , and Pot1a gene expression ( P  
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We revealed that the Tert , Terc , Trf1 , Trf2 , and Pot1a gene expression ( P  &lt; 0.001) and TERT protein ( P  &lt; 0.01) levels significantly decreased in certain ovarian follicles of the aged group when compared to those of the adult group. Also, telomerase activity exhibited remarkable changes in the follicles of both groups. Consequently, altered telomere-associated gene expression and reduced TERT protein levels in the follicles of aged mice may be a determinant of telomere shortening during ovarian aging, and infertility appearing in the later decades of reproductive lifespan. 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subjects 631/80
631/80/509
Aging
Chromosomes
DNA biosynthesis
Embryos
Fertility
Follicles
Gene expression
Genotoxicity
Humanities and Social Sciences
Infertility
Life span
Molecular modelling
multidisciplinary
Ovaries
Proteins
RNA-directed DNA polymerase
Science
Science (multidisciplinary)
Stem cell transplantation
Stem cells
Telomerase
Telomerase reverse transcriptase
Telomere-binding protein
Telomeres
TRF2 protein
title Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice
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