Functional distribution of synapsin I in human sperm

• Published in: FEBS open bio 2015-01, Vol.5 (1), p.801-808
• Main Authors: Coleman, William L., Kulp, Adam C., Venditti, Jennifer J.
• Format: Article
• Language: English
• Subjects: Acrosome reaction; Antibodies; Brain research; Capacitation; Cell interactions; Communication; Epididymis; Equatorial segment; Exocytosis; Human sperm; Immunocytochemistry; Immunoglobulins; Kinases; Localization; Membranes; Motility; Neurons; Phosphorylation; Proteins; Research article; Semen; Sperm; Studies; Synapsin; Synapsin I
• ISSN: 2211-5463; 2211-5463
• DOI: 10.1016/j.fob.2015.09.006

•Synapsin I was localized in the human sperm equatorial segment.•Presence of synapsin I was confirmed by dot and Western blotting techniques.•Treatment of sperm with anti-synapsin antibodies significantly decreased motility. Proteins known to function during cell–cell communication and exocytosis in neurons and other secretory cells have recently been reported in human sperm. Synapsins are a group of proteins that have been very well characterized in neurons, but little is known about synapsin function in other cell types. Based upon previous findings and the known function of synapsin, we tested the hypothesis that synapsin I was present in human sperm. Washed, capacitated, and acrosome induced sperm preparations were used to evaluate the functional distribution of synapsin I using immunocytochemistry. Protein extracts from mouse brain, mouse testis/epididymis, and human semen were used for protein blotting techniques. Immunolocalization revealed synapsin I was enriched in the sperm equatorial segment. Protein extracts from mouse brain, mouse testis/epididymis, and human semen were positive for synapsin I using several different antibodies, and dot blot results were confirmed by Western blot analyses. Finally, treatment of capacitated and acrosome reaction induced samples with anti-synapsin antibodies significantly reduced sperm motility. Localization of synapsin I in human sperm is a novel finding. The association of synapsin I with the sperm equatorial segment and effects on motility are suggestive of a role associated with capacitation and/or acrosome reaction, processes that render sperm capable of fertilizing an oocyte.