The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering

Cohesin tethers together regions of DNA, thereby mediating higher order chromatin organization that is critical for sister chromatid cohesion, DNA repair and transcriptional regulation. Cohesin contains a heterodimeric ATP-binding Cassette (ABC) ATPase comprised of Smc1 and Smc3 ATPase active sites....

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Published in:eLife 2015-11, Vol.4
Main Authors: Çamdere, Gamze, Guacci, Vincent, Stricklin, Jeremiah, Koshland, Douglas
Format: Article
Language:English
Subjects:
ABC-binding cassette
Acetylation
Acetyltransferase
Acetyltransferases - metabolism
Adenosine triphosphatase
Adenosine Triphosphatases - metabolism
ATPase
Biochemistry
Cell Biology
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell research
Cellular control mechanisms
Cellular proteins
Chromatin
Chromatin - metabolism
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
Chromosomes
Cloning
Cohesin
Cohesins
Deoxyribonucleic acid
DNA
DNA - metabolism
DNA repair
Experiments
Gene expression
Gene regulation
Genetic aspects
Mutation
Nuclear Proteins - metabolism
Physiological aspects
Plasmids
Proteins
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Standard deviation
structural maintenance of chromosomes
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Summary:Cohesin tethers together regions of DNA, thereby mediating higher order chromatin organization that is critical for sister chromatid cohesion, DNA repair and transcriptional regulation. Cohesin contains a heterodimeric ATP-binding Cassette (ABC) ATPase comprised of Smc1 and Smc3 ATPase active sites. These ATPases are required for cohesin to bind DNA. Cohesin's DNA binding activity is also promoted by the Eco1 acetyltransferase and inhibited by Wpl1. Recently we showed that after cohesin stably binds DNA, a second step is required for DNA tethering. This second step is also controlled by Eco1 acetylation. Here, we use genetic and biochemical analyses to show that this second DNA tethering step is regulated by cohesin ATPase. Furthermore, our results also suggest that Eco1 promotes cohesion by modulating the ATPase cycle of DNA-bound cohesin in a state that is permissive for DNA tethering and refractory to Wpl1 inhibition.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.11315