De Novo Transcriptome Analysis of the Lizard Fish (Saurida elongata): Novel Insights into Genes Related to Sex Differentiation

Among vertebrates, teleost fishes exhibit the largest array of sex-determining systems, resulting in many reproductive strategies. Screening these fish for sex-related genes could enhance our understanding of sexual differentiation. The lizardfish, Saurida elongata (Temminck & Schlegel, 1846), i...

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Bibliographic Details
Published in:Applied sciences 2022-11, Vol.12 (22), p.11319
Main Authors: Shan, Binbin, Wang, Liangming, Liu, Yan, Yang, Changping, Liu, Manting, Sun, Dianrong, Huang, Pujiang
Format: Article
Language:English
Subjects:
Annotations
Aquaculture
demersal fish
differentially expressed genes
Differentiation
Elongation
Females
Fish
Fishing
gene ontology
Genes
Genomes
Genomics
Marine fish
Proteins
RNA-seq
Saurida elongata
Sex differentiation
Sexes
Signal transduction
Simple sequence repeats
Software
synodontidae
Transcriptomes
Vertebrates
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Summary:Among vertebrates, teleost fishes exhibit the largest array of sex-determining systems, resulting in many reproductive strategies. Screening these fish for sex-related genes could enhance our understanding of sexual differentiation. The lizardfish, Saurida elongata (Temminck & Schlegel, 1846), is a commercially important marine fish in tropical and subtropical seas of the northwest Pacific. However, little genomic information on S. elongata is available. In this study, the transcriptomes of three female and three male S. elongata were sequenced. A total of 49.19 million raw read pairs were generated. After identification and assembly, a total of 59,902 nonredundant unigenes were obtained with an N50 length of 2070 bp. Then, 38,016 unigenes (63.47% of the total) were successfully annotated through multiple public databases. A comparison of the unigenes of different sexes of S. elongata revealed that 22,507 unigenes (10,419 up-regulated in a female and 12,088 up-regulated in a male) were differentially expressed between sexes. Then, numerous candidate sex-related genes were identified, including dmrt2, dmrt4, foxl2, zps and starts. Furthermore, 23,941 simple sequence repeats (SSRs) were detected in SSR-containing sequences. This informative transcriptome analysis provides valuable data to increase the genomic resources of S. elongata.
ISSN:2076-3417
2076-3417
DOI:10.3390/app122211319