Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots

In plants, flooding-induced oxygen deficiency causes severe stress, leading to growth reduction and yield loss. It is therefore important to understand the molecular mechanisms for adaptation to hypoxia. Aquaporins at the plasma membrane play a crucial role in water uptake. However, their role durin...

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Bibliographic Details
Published in:Antioxidants 2022-04, Vol.11 (5), p.836
Main Authors: Hofmann, Anne, Wienkoop, Stefanie, Lüthje, Sabine
Format: Article
Language:English
Subjects:
Abiotic stress
Alcohol dehydrogenase
antioxidant
aquaporin
Aquaporins
Cytochrome
Electron transport
electron transport system
Ethylene
Flooding
Homeostasis
Hydrogen peroxide
Hypoxia
Mass spectroscopy
Molecular modelling
Oxygen
Physiology
plant growth regulators
plasma membrane
Plasma membranes
Proteins
Proteomes
Proteomics
Stress response
Transcriptomics
Water uptake
Zea mays
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Summary:In plants, flooding-induced oxygen deficiency causes severe stress, leading to growth reduction and yield loss. It is therefore important to understand the molecular mechanisms for adaptation to hypoxia. Aquaporins at the plasma membrane play a crucial role in water uptake. However, their role during hypoxia and membrane redox changes is still not fully understood. The influence of 24 h hypoxia induction on hydroponically grown maize ( L.) was investigated using an oil-based setup. Analyses of physiological parameters revealed typical flooding symptoms such as increased ethylene and H O levels, an increased alcohol dehydrogenase activity, and an increased redox activity at the plasma membrane along with decreased oxygen of the medium. Transcriptomic analysis and shotgun proteomics of plasma membranes and soluble fractions were performed to determine alterations in maize roots. RNA-sequencing data confirmed the upregulation of genes involved in anaerobic metabolism, biosynthesis of the phytohormone ethylene, and its receptors. Transcripts of several antioxidative systems and other oxidoreductases were regulated. Mass spectrometry analysis of the plasma membrane proteome revealed alterations in redox systems and an increased abundance of aquaporins. Here, we discuss the importance of plasma membrane aquaporins and redox systems in hypoxia stress response, including the regulation of plant growth and redox homeostasis.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox11050836