Evaluation of Resistance Induction Promoted by Bioactive Compounds of Pseudomonas aeruginosa LV Strain against Asian Soybean Rust

• Published in: Microorganisms (Basel) 2024-08, Vol.12 (8), p.1576
• Main Authors: Barazetti, André Riedi, Dealis, Mickely Liuti, Basso, Kawany Roque, Silva, Maria Clara Davis, Alves, Leonardo da Cruz, Parra, Maria Eugênia Alcântara, Simionato, Ane Stéfano, Cely, Martha Viviana Torres, Macedo, Arthur Ladeira, Silva, Denise Brentan, Andrade, Galdino
• Format: Article
• Language: English
• Subjects: Agricultural production; antimicrobial; Antimicrobial agents; Bacteria; Bioactive compounds; Biosynthesis; Chromatography; Crop production; Crop yield; Design of experiments; Experimental design; Fatty acids; flavonoid; Flavonoids; Fungi; Fungicides; Genistein; Isoflavones; Kaempferol; Leaves; Metabolites; metabolomic; Microorganisms; Pathogens; Peptides; Pesticides; Phakopsora pachyrhizi; phenazine; Plant bacterial diseases; Plant growth; Plant growth promoters; Plant resistance; Pseudomonas aeruginosa; Reactive oxygen species; Rust fungi; Saponins; Secondary metabolites; Seeds; Soybean; Soybean industry; Soybeans; Sphingolipids; Wildlife conservation
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms12081576

are known as higher producers of secondary metabolites with antimicrobial properties and plant growth promoters, including resistance induction. These mechanisms should be an alternative to pesticide use in crop production. causes Asian soybean rust, representing a high loss of yield around the world. The objective of this paper was to evaluate the application of secondary metabolites produced by LV strain from the semi-purified fraction F4A in soybean plants to induce plant resistance against in field conditions. The experimental design was performed in randomized blocks with three replicates using two F4A doses (1 and 10 μg mL ) combined or not with fungicides (Unizeb Gold or Sphere Max ). The control treatment, with Uni + Sph, saponins, flavonoids, and sphingolipids, showed higher intensities in the plants. In contrast, plants treated with the F4A fraction mainly exhibited fatty acid derivatives and some non-identified compounds with nitrogen. Plants treated with Sphere Max , with or without F4A10, showed higher intensities of glycosylated flavonoids, such as kaempferol, luteolin, narigenin, and apigenin. Plants treated with F4A showed higher intensities of genistein and fatty acid derivatives. These increases in flavonoid compound biosynthesis and antioxidant properties probably contribute to the protection against reactive oxygen species (ROS).