Design of a Haversian system-like gradient porous scaffold based on triply periodic minimal surfaces for promoting bone regeneration

• Published in: Journal of advanced research 2023-12, Vol.54, p.89-104
• Main Authors: Li, Lan, Wang, Peng, Liang, Huixin, Jin, Jing, Zhang, Yibo, Shi, Jianping, Zhang, Yun, He, Siyuan, Mao, Hongli, Xue, Bin, Lai, Jiancheng, Zhu, Liya, Jiang, Qing
• Format: Article
• Language: English
• Subjects: 3D printing; Bone Regeneration; Bone tissue engineering; Haversian System; Osteogenesis; Pore geometry; Porosity; Porous scaffold; Tissue Scaffolds - chemistry; Triply periodic minimal surface
• ISSN: 2090-1232; 2090-1224
• DOI: 10.1016/j.jare.2023.01.004

[Display omitted] •The Haversian system-like gradient scaffolds using the TPMS structure realized bionics of the natural bone tissue.•The gradient TPMS scaffold demonstrated appropriate shear stress and larger permeability, leading to better bone ingrowth.•The gradient TPMS scaffold exhibited better performance in stress conduction and stress shielding effect can be reduced.•The in silico model can do benefit for the future scaffold design by reducing time cost and animal numbers. The bone ingrowth depth in the porous scaffolds is greatly affected by the structural design, notably the pore size, pore geometry, and the pore distribution. To enhance the bone regeneration capability of scaffolds, the bionic design can be regarded as a potential solution. We proposed a Haversian system-like gradient structure based on the triply periodic minimal surface architectures with pore size varying from the edge to the center. And its effects in promoting bone regeneration were evaluated in the study. The gradient scaffold was designed using the triply periodic minimal surface architectures. The mechanical properties were analyzed by the finite element simulation and confirmed using the universal machine. The fluid characteristics were calculated by the computational fluid dynamics analysis. The bone regeneration process was simulated using a in silico computational model containing the main biological, physical, and chemical variation during the bone growth process. Finally, the in vitro and in vivo studies were carried out to verify the actual osteogenic effect. Compared to the uniform scaffold, the biomimetic gradient scaffold demonstrated better performance in stress conduction and reduced stress shielding effects. The fluid features were appropriate for cell migration and flow diffusion, and the permeability was in the same order of magnitude with the natural bone. The bone ingrowth simulation exhibited improved angiogenesis and bone regeneration. Higher expression of the osteogenesis-related genes, higher alkaline phosphatase activity, and increased mineralization could be observed on the gradient scaffold in the in vitro study. The 12-week in vivo study proved that the gradient scaffold had deeper bone inserting depth and a more stable bone-scaffold interface. The Haversian system-like gradient structure can effectively promote the bone regeneration. This structural design can be used as a new solution for the clinical application of prosthesis design.