Is Acrylamide as Harmful as We Think? A New Look at the Impact of Acrylamide on the Viability of Beneficial Intestinal Bacteria of the Genus Lactobacillus

The impact of acrylamide (AA) on microorganisms is still not clearly understood as AA has not induced mutations in bacteria, but its epoxide analog has been reported to be mutagenic in strains. The aim of the study was to evaluate whether AA could influence the growth and viability of beneficial int...

Full description

Saved in:
Bibliographic Details
Published in:Nutrients 2020-04, Vol.12 (4), p.1157
Main Authors: Petka, Katarzyna, Tarko, Tomasz, Duda-Chodak, Aleksandra
Format: Article
Language:English
Subjects:
Acrylamide
Acrylamide - adverse effects
Acrylamide - analysis
Acrylamide - toxicity
Bacteria
Cell culture
Cell division
Cereals
Colonization
Cytology
Deoxyribonucleic acid
DNA
DNA damage
E coli
Evaluation
Flow cytometry
Food Analysis
Food Handling
Food production
Food products
Gastrointestinal Microbiome - drug effects
Glycation End Products, Advanced
Humans
Intestine
Laboratories
Lactic acid
Lactic acid bacteria
Lactobacilli
Lactobacillus acidophilus - drug effects
Lactobacillus acidophilus - growth & development
Lactobacillus brevis - drug effects
Lactobacillus brevis - growth & development
Lactobacillus plantarum - drug effects
Lactobacillus plantarum - growth & development
Microbiota
Microorganisms
Mutation
probiotic
Probiotics
Salmonella
Viability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The impact of acrylamide (AA) on microorganisms is still not clearly understood as AA has not induced mutations in bacteria, but its epoxide analog has been reported to be mutagenic in strains. The aim of the study was to evaluate whether AA could influence the growth and viability of beneficial intestinal bacteria. The impact of AA at concentrations of 0-100 µg/mL on lactic acid bacteria (LAB) was examined. Bacterial growth was evaluated by the culture method, while the percentage of alive, injured, and dead bacteria was assessed by flow cytometry after 24 h and 48 h of incubation. We demonstrated that acrylamide could influence the viability of the LAB, but its impact depended on both the AA concentration and the bacterial species. The viability of probiotic strain LA-5 increased while that of decreased; was less sensitive. Moreover, AA influenced the morphology of probably by blocking cell separation during division. We concluded that acrylamide present in food could modulate the viability of LAB and, therefore, could influence their activity in food products or, after colonization, in the human intestine.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu12041157