Anisodamine ameliorates crystalline silica-exposed pulmonary inflammation and fibrosis via the α7nAChR/JAK2/STAT3 signaling pathway

• Published in: Ecotoxicology and environmental safety 2025-01, Vol.289, p.117534, Article 117534
• Main Authors: Liu, Meng, Liu, Hui, Kang, Hong, Wu, Juan, Xing, Puhua, Ding, Xiaorui, Wei, Yangyang, Kong, Xiaomei
• Format: Article
• Language: English
• Subjects: Anisodamine; JAK2/STAT3, Inflammation; Silicosis; α7nAChR
• ISSN: 0147-6513; 1090-2414; 1090-2414
• DOI: 10.1016/j.ecoenv.2024.117534

Silicosis is a systemic disease marked by diffuse pulmonary fibrosis resulting from prolonged inhalation of crystalline silica (CS) dust. This study aimed to examine the effects of anisodamine (ANI) on pulmonary inflammation and fibrosis in silicosis, as well as to elucidate the underlying molecular mechanisms. Animal experiments demonstrated that ANI significantly reduced alveolar structure damage and the formation of silicosis nodules in affected mice, as confirmed by pathological slides. ANI inhibited the expression of tumor necrosis factor (TNF-α) in bronchoalveolar lavage fluid (BALF) while promoting the secretion of interleukin-4 (IL-4) and interleukin-10 (IL-10). Further molecular investigations indicated a strong link between pulmonary inflammation and fibrosis, showing decreased levels of α7nAChR and increased expression of phosphorylated Janus kinase 2 (JAK2) and phosphorylated transcription factor 3 (STAT3) in the lung tissues of mice exposed to CS. The relevant molecular alterations in the lung tissue of the model group of mice were reversed by ANI. Methyllycaconitine(MLA, α7nAChR inhibitor) and RO8191 (JAK2/STAT3 agonist) could reverse the therapeutic effect of ANI in silicosis and related molecular mechanisms. The results suggest that ANI may alleviate silicosis by inhibiting pulmonary inflammation and fibrosis through modulation of the JAK2/STAT3 signaling pathway, which is mediated by α7nAChR. Coal workers can utilize ANI early on to treat and prevent silicosis. •ANI could reduce the silica-induced pulmonary inflammation and fibrosis and inhibit the progression of silicosis.•ANI inhibited silicosis lung inflammation through the α7nAChR/JAK2/STAT3 pathway and further suppressed pulmonary fibrosis.•Early intervention of ANI could be effective in exerting anti-inflammatory effects, and the findings provide guidance for the treatment of silicosis.