Diversity of metallo-β-lactamase-encoding genes found in distinct species of Acinetobacter isolated from the Brazilian Amazon Region

The multidrug resistance (MDR) phenotype is frequently observed in Acinetobacter baumannii, the most clinically relevant pathogenic species of its genus; recently, other species belonging to the A. calcoaceticus-A. baumannii complex have emerged as important MDR nosocomial pathogens. The present stu...

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Published in:Memórias do Instituto Oswaldo Cruz 2019-01, Vol.114, p.e190020-e190020
Main Authors: Brasiliense, Danielle, Cayô, Rodrigo, Streling, Ana Paula, Nodari, Carolina S, Barata, Rafael R, Lemos, Poliana S, Massafra, Janaina M, Correa, Yan, Magalhães, Igor, Gales, Ana C, Sodré, Roberta
Format: Article
Language:English
Subjects:
ICU
metallo-β-lactamase
non-baumannii Acinetobacter species
nosocomial infection
Original
PARASITOLOGY
polymyxin resistance
Tn125
TROPICAL MEDICINE
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Summary:The multidrug resistance (MDR) phenotype is frequently observed in Acinetobacter baumannii, the most clinically relevant pathogenic species of its genus; recently, other species belonging to the A. calcoaceticus-A. baumannii complex have emerged as important MDR nosocomial pathogens. The present study aimed to verify the occurrence of metallo-β-lactamase genes among distinct Acinetobacter species in a hospital located in the Brazilian Amazon Region. Antimicrobial susceptibility profiles were determined by broth microdilution. The genetic relationships among these isolates were assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Pyrosequencing reads of plasmids carrying the bla NDM-1 gene were generated using the Ion Torrent™ platform sequencing. A total of six isolates carried bla NDM-1: A. baumannii (n = 2), A. nosocomialis (n = 3), and A. pittii (n = 1); three carried bla IMP-1: A. baumannii, A. nosocomialis, and A. bereziniae. Resistance to colistin was observed for an NDM-1-producing A. nosocomialis isolate. Diverse PFGE patterns and sequence types were found among A. nosocomialis and A. baumannii isolates. The bla NDM-1 sequence was inserted in a Tn125 transposon, while the bla IMP-1 was found as a gene cassette of the class 1 integron In86. To the best of our knowledge, this is the first report describing the dissemination of bla NDM-1 among distinct Acinetobacter species recovered from the same hospital in South America.
ISSN:0074-0276
1678-8060
1678-8060
DOI:10.1590/0074-02760190020