Modulation of Membrane Trafficking of AQP5 in the Lens in Response to Changes in Zonular Tension Is Mediated by the Mechanosensitive Channel TRPV1

In mice, the contraction of the ciliary muscle via the administration of pilocarpine reduces the zonular tension applied to the lens and activates the TRPV1-mediated arm of a dual feedback system that regulates the lens' hydrostatic pressure gradient. In the rat lens, this pilocarpine-induced r...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2023-05, Vol.24 (10), p.9080
Main Authors: Petrova, Rosica S, Nair, Nikhil, Bavana, Nandini, Chen, Yadi, Schey, Kevin L, Donaldson, Paul J
Format: Article
Language:English
Subjects:
Animals
AQP5
Aquaporin 5
Aquaporins
Capsaicin receptors
Cell division
Efflux
Hydrostatic pressure
lens
lens surface pressure
Lens, Crystalline
Membrane trafficking
Membranes
Mice
Microelectrodes
Muscle contraction
Osmosis
Permeability
Pilocarpine
Pilocarpine - pharmacology
Pressure
Proteins
Rats
Sutures
TRPV Cation Channels
TRPV1
water transport
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In mice, the contraction of the ciliary muscle via the administration of pilocarpine reduces the zonular tension applied to the lens and activates the TRPV1-mediated arm of a dual feedback system that regulates the lens' hydrostatic pressure gradient. In the rat lens, this pilocarpine-induced reduction in zonular tension also causes the water channel AQP5 to be removed from the membranes of fiber cells located in the anterior influx and equatorial efflux zones. Here, we determined whether this pilocarpine-induced membrane trafficking of AQP5 is also regulated by the activation of TRPV1. Using microelectrode-based methods to measure surface pressure, we found that pilocarpine also increased pressure in the rat lenses via the activation of TRPV1, while pilocarpine-induced removal of AQP5 from the membrane observed using immunolabelling was abolished by pre-incubation of the lenses with a TRPV1 inhibitor. In contrast, mimicking the actions of pilocarpine by blocking TRPV4 and then activating TRPV1 resulted in sustained increase in pressure and the removal of AQP5 from the anterior influx and equatorial efflux zones. These results show that the removal of AQP5 in response to a decrease in zonular tension is mediated by TRPV1 and suggest that regional changes to P contribute to lens hydrostatic pressure gradient regulation.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24109080