International Mycoplasma pneumoniae typing study: interpretation of M. pneumoniae multilocus variable-number tandem-repeat analysis

Abstract Typing of Mycoplasma pneumoniae by multiple-locus variable-number tandem repeat analysis (MLVA) is increasingly in use. However, no specific internationally agreed guidance is available. Thirty M. pneumoniae DNA samples including serial dilutions of a type strain were sent to six internatio...

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Bibliographic Details
Published in:New microbes and new infections 2015-09, Vol.7 (C), p.37-40
Main Authors: Chalker, V.J, Pereyre, S, Dumke, R, Winchell, J, Khosla, P, Sun, H, Yan, C, Vink, C, Bébéar, C
Format: Article
Language:English
Subjects:
Infectious Disease
Interpretation guidelines
molecular typing
multiple-locus variable-number tandem repeat analysis (MLVA)
Mycoplasma pneumoniae
New Microbes in Humans
standardisation
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Summary:Abstract Typing of Mycoplasma pneumoniae by multiple-locus variable-number tandem repeat analysis (MLVA) is increasingly in use. However, no specific internationally agreed guidance is available. Thirty M. pneumoniae DNA samples including serial dilutions of a type strain were sent to six international laboratories to perform MLVA and results were compared. Good correlation was observed, indicating that this methodology can be robustly performed in multiple sites. However, differences due to interpretation of fragment size, repeat sequence identification and repeat numbering led to inconsistency in the final profiles assigned by laboratories. We propose guidelines for interpreting M. pneumoniae MLVA typing and assigning the number of repeats.
ISSN:2052-2975
2052-2975
DOI:10.1016/j.nmni.2015.05.005