How Does the Concentration of Technetium-99m Radiolabeled Gold Nanoparticles Affect Their In Vivo Biodistribution?

Gold nanoparticles (AuNPs) radiolabeled with therapeutic and diagnostic radioisotopes have been broadly studied as a promising platform for early diagnosis and treatment of many diseases including cancer. Our main goal for this study was the comparison of the biodistribution profiles of four differe...

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Bibliographic Details
Published in:Applied sciences 2024-05, Vol.14 (10), p.4324
Main Authors: Apostolopoulou, Adamantia, Salvanou, Evangelia-Alexandra, Chiotellis, Aristeidis, Pirmettis, Nektarios N, Pirmettis, Ioannis C, Xanthopoulos, Stavros, Koźmiński, Przemysław, Bouziotis, Penelope
Format: Article
Language:English
Subjects:
Biodistribution
Blood
Cancer
Cysteine
cytotoxicity
gold nanoparticles
Histidine
Kidneys
Liver
Nanoparticles
Oncology, Experimental
radiolabeling
Spleen
technetium-99m
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Summary:Gold nanoparticles (AuNPs) radiolabeled with therapeutic and diagnostic radioisotopes have been broadly studied as a promising platform for early diagnosis and treatment of many diseases including cancer. Our main goal for this study was the comparison of the biodistribution profiles of four different concentrations of gold nanoconjugates radiolabeled with Technetium-99m (99mTc). More specifically, AuNPs with an average diameter of 2 nm were functionalized with a tridentate thiol ligand. Four different concentrations were radiolabeled with 99mTc-tricarbonyls with high radiolabeling yields (>85%) and were further purified, leading to radiochemical purity of >95%. In vitro stability of the radiolabeled nanoconstructs was examined in cysteine and histidine solutions as well as in human serum, exhibiting robust radiolabeling up to 24 h post-preparation. Moreover, in vitro cytotoxicity studies were carried out in 4T1 murine mammary cancer cells. In vivo tracking of the radiolabeled nanoconjugates at both concentrations was examined in normal mice in order to examine the effect of AuNPs’ concentration on their in vivo kinetics. Our work demonstrates that varying concentrations of radiolabeled AuNPs lead to notably different biodistribution profiles.
ISSN:2076-3417
2076-3417
DOI:10.3390/app14104324