Generation of induced pluripotent stem cells (IBMSi011-A) from a patient with Parkinson's disease carrying LRRK2 p.I1371V mutation

Leucine rich repeat kinase 2 (LRRK2) is the causative gene for autosomal-dominant familial forms of Parkinson's disease (PD). Here, we generated induced pluripotent stem cells (iPSCs) from the peripheral blood mononuclear cells of a female patient with LRRK2 c.4111A > G (p.I1371V) mutation b...

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Bibliographic Details
Published in:Stem cell research 2019-05, Vol.37, p.101447-101447, Article 101447
Main Authors: Lin, Han-I, Cheng, Yu-Che, Ko, Hui-Wen, Wen, Cheng-Hao, Lu, Huai-En, Huang, Ching-Ying, Hsieh, Patrick C.H., Lin, Chin-Hsien
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Cells, Cultured
Cellular Reprogramming
Female
Humans
Induced Pluripotent Stem Cells - metabolism
Induced Pluripotent Stem Cells - pathology
Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 - genetics
Leukocytes, Mononuclear - metabolism
Leukocytes, Mononuclear - pathology
Mice
Mice, Inbred NOD
Mice, SCID
Middle Aged
Mutation
Parkinson Disease - genetics
Parkinson Disease - pathology
Phenotype
Teratoma - etiology
Teratoma - pathology
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Summary:Leucine rich repeat kinase 2 (LRRK2) is the causative gene for autosomal-dominant familial forms of Parkinson's disease (PD). Here, we generated induced pluripotent stem cells (iPSCs) from the peripheral blood mononuclear cells of a female patient with LRRK2 c.4111A > G (p.I1371V) mutation by using the Sendai-virus delivery system. The resulting iPSCs had a normal karyotype. The iPSCs also showed pluripotency confirmed by immunofluorescent staining and differentiated into the three germ layers in vivo. This cellular model will provide a platform for studying the role of LRRK2 in the disease process.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2019.101447