Absorption, translocation, and effects of Bt Cry1Ac peptides from transgenic cotton to the intercrops and soil functional bacteria

• Published in: Scientific reports 2020-10, Vol.10 (1), p.17294-11, Article 17294
• Main Authors: Zhang, Wei, Cao, Zhen, Wang, Mian, Chen, Xiaojiao, Wang, Baomin
• Format: Article
• Language: English
• Subjects: 631/45; 631/61; Bacillus thuringiensis - genetics; Bacillus thuringiensis Toxins - metabolism; China; Crops, Agricultural - growth & development; Crops, Agricultural - metabolism; Endotoxins - metabolism; Gossypium - metabolism; Gossypium - microbiology; Hemolysin Proteins - metabolism; Humanities and Social Sciences; Hydrolysis; multidisciplinary; Nitrogen Fixation; Organophosphates - metabolism; Plants, Genetically Modified - metabolism; Plants, Genetically Modified - microbiology; Science; Science (multidisciplinary); Soil Microbiology; Streptomyces griseus - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-73375-8

Insecticidal proteins encoded by the truncated genes from Bacillus thuringiensis ( Bt ) in transgenic crops are released into soil mainly through root exudate and crop residues. In the present study, Bt Cry1Ac protein was hydrolyzed by pronase that was secreted by the soil bacterium Streptomyces griseus . Six peptides were identified as the products of enzymatic hydrolysis by nano liquid chromatography tandem mass spectrometry (LC–MS/MS). One of the six peptides was labeled with radioactive isotope iodine-125 and then purified. The 125 I-peptide solution was irrigated to the rhizosphere soil of watermelon seedlings ( Citrullus lanatus L.) and wheat seedlings ( Triticum aestivum L.), which the two crops usually intercrop with cotton in China. Detection of radioactivity in both plant tissues within one hour proved adsorption, uptake and translocation of the peptide into watermelon and wheat seedlings. Three of the identified peptides were sprayed onto the seedling leaves of watermelon, wheat and maize ( Zea mays L.) in the field or the growth chamber. No significant effects on plant growth were observed. These peptides also did not affect growth of organic phosphate-dissolving, nitrogen-fixing, and potassium-dissolving bacteria in the culture. This study provides a new view of GMO risk assessment methodology.