Contamination of Animal Feed with Undeclared Tetracyclines-Confirmatory Analysis by Liquid Chromatography-Mass Spectrometry after Microbiological Plate Test

The presence of tetracycline (TC) antibiotics was determined in animal feed that had been previously screened with a microbiological plate test. Feed samples were screened by a microbiological plate test on a pH 6.0 culture medium seeded with ATCC 11778 able to pre-reveal the presence of tetracyclin...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2020-05, Vol.25 (9), p.2162
Main Authors: Przeniosło-Siwczyńska, Monika, Patyra, Ewelina, Grelik, Aleksandra, Chyłek-Purchała, Maja, Kozak, Beata, Kwiatek, Krzysztof
Format: Article
Language:English
Subjects:
Animal feed
Animal Feed - microbiology
Animals
Antibiotics
Antimicrobial agents
Bacillus cereus - isolation & purification
Bacteria
Bacterial infections
Breeding of animals
Cattle
Chlortetracycline
Chlortetracycline - analysis
Chromatography
Chromatography, Liquid
Contamination
Doxycycline
Doxycycline - analysis
Drug Residues - analysis
feed analysis
Feeds
Food contamination & poisoning
Food Contamination - analysis
High performance liquid chromatography
LC−MS
Liquid chromatography
Mass Spectrometry
Mass spectroscopy
Methods
microbial screening assay
Poultry
Scientific imaging
Swine
Tetracyclines
Tetracyclines - analysis
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Summary:The presence of tetracycline (TC) antibiotics was determined in animal feed that had been previously screened with a microbiological plate test. Feed samples were screened by a microbiological plate test on a pH 6.0 culture medium seeded with ATCC 11778 able to pre-reveal the presence of tetracyclines. Subsequently, confirmation and quantification were performed using a validated HPLC method with mass spectrometric detection. In 2013-2018, 353 feed samples were analysed to detect antibacterial substances, of which 186 (52.7%) were suspected to contain tetracyclines. Forty-two out of 186 (22.6%) samples analysed by the chromatographic method contained undeclared tetracyclines, which were determined at concentrations from 0.3 to 49 mg kg . The most frequently identified contaminating tetracyclines were doxycycline and chlortetracycline.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules25092162