Soluble expression of recombinant coagulation factor IX protein using Escherichia coli

Hemophilia B is a congenital bleeding disorder caused by factor IX (FIX) deficiency. Generation of recombinant FIX (rFIX) is required for detecting a Hemophilia B indicator, anti-FIX antibody. In this study, we described a method for producing recombinant FIX (rFIX) using Escherichia coli. We constr...

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Bibliographic Details
Published in:Biochemistry and biophysics reports 2024-07, Vol.38, p.101714-101714, Article 101714
Main Authors: Park, Byoung-Hee, Yun, Hanool, Jeong, Hee-Jin
Format: Article
Language:English
Subjects:
Escherichia coli
Factor IX
Hemophilia
Protein expression
Recombinant protein
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Summary:Hemophilia B is a congenital bleeding disorder caused by factor IX (FIX) deficiency. Generation of recombinant FIX (rFIX) is required for detecting a Hemophilia B indicator, anti-FIX antibody. In this study, we described a method for producing recombinant FIX (rFIX) using Escherichia coli. We constructed a FIX-expressing plasmid without a fusion tag protein-encoding gene and produced rFIX as a soluble form within five days. Dose-dependent curve was obtained from ELISA using anti-FIX antibody, indicating that the rFIX can be used as an antigen to detect anti-FIX antibody with high affinity and sensitivity. •Generation of a recombinant factor IX (rFIX) to compensate for detecting anti-FIX antibody in patients with hemophilia B.•Expression of a soluble form of full-length rFIX with no fusion sequence.•Confirmation of the binding efficiency of rFIX to anti-FIX antibody.
ISSN:2405-5808
2405-5808
DOI:10.1016/j.bbrep.2024.101714