Soluble expression of recombinant coagulation factor IX protein using Escherichia coli

Hemophilia B is a congenital bleeding disorder caused by factor IX (FIX) deficiency. Generation of recombinant FIX (rFIX) is required for detecting a Hemophilia B indicator, anti-FIX antibody. In this study, we described a method for producing recombinant FIX (rFIX) using Escherichia coli. We constr...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry and biophysics reports 2024-07, Vol.38, p.101714-101714, Article 101714
Main Authors: Park, Byoung-Hee, Yun, Hanool, Jeong, Hee-Jin
Format: Article
Language:English
Subjects:
Escherichia coli
Factor IX
Hemophilia
Protein expression
Recombinant protein
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c420t-fbd620ae6e8ff1e066e18d1285aa1c74d8fe637c372c3f6f600b2c176b7824363
container_end_page 101714
container_issue
container_start_page 101714
container_title Biochemistry and biophysics reports
container_volume 38
creator Park, Byoung-Hee
Yun, Hanool
Jeong, Hee-Jin
description Hemophilia B is a congenital bleeding disorder caused by factor IX (FIX) deficiency. Generation of recombinant FIX (rFIX) is required for detecting a Hemophilia B indicator, anti-FIX antibody. In this study, we described a method for producing recombinant FIX (rFIX) using Escherichia coli. We constructed a FIX-expressing plasmid without a fusion tag protein-encoding gene and produced rFIX as a soluble form within five days. Dose-dependent curve was obtained from ELISA using anti-FIX antibody, indicating that the rFIX can be used as an antigen to detect anti-FIX antibody with high affinity and sensitivity. •Generation of a recombinant factor IX (rFIX) to compensate for detecting anti-FIX antibody in patients with hemophilia B.•Expression of a soluble form of full-length rFIX with no fusion sequence.•Confirmation of the binding efficiency of rFIX to anti-FIX antibody.
doi_str_mv 10.1016/j.bbrep.2024.101714
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_5b74baad28674a01bf7713b9c94f5c5b</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S2405580824000785</els_id><doaj_id>oai_doaj_org_article_5b74baad28674a01bf7713b9c94f5c5b</doaj_id><sourcerecordid>3048494510</sourcerecordid><originalsourceid>FETCH-LOGICAL-c420t-fbd620ae6e8ff1e066e18d1285aa1c74d8fe637c372c3f6f600b2c176b7824363</originalsourceid><addsrcrecordid>eNp9kU1PGzEQhq2KChDlFyBVe-SSMP5Y2zn0gBAtkZB66Id6s2zvODjarFN7F9F_X4elqCdOtsbvvO-MH0IuKCwpUHm1XTqXcb9kwMShoqh4R06ZgHbRatBH_91PyHkpWwCgLdMtk8fkhGupqVT0lPz8lvrJ9djg0z5jKTENTQpNRp92Lg52GBuf7Gbq7Xh4CtaPKTfrX80-pxHj0EwlDpvmtvgHzNE_RFv1ffxA3gfbFzx_Oc_Ij8-332_uFvdfv6xvru8XXjAYF8F1koFFiToEiiAlUt3ROqa11CvR6YCSK88V8zzIIAEc81RJpzQTXPIzsp59u2S3Zp_jzuY_Jtlongspb4zNY_Q9mtYp4aztmJZKWKAuKEW5W_mVCK1vXfW6nL3qar8nLKPZxeKx7-2AaSqGg9BiJVoKVcpnqc-plIzhNZqCOfAxW_PMxxz4mJlP7fr4EjC5HXavPf9oVMGnWYD1yx4jZlN8xMFjFyuQse4U3wz4CzoaocQ</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3048494510</pqid></control><display><type>article</type><title>Soluble expression of recombinant coagulation factor IX protein using Escherichia coli</title><source>PubMed Central Free</source><source>ScienceDirect®</source><creator>Park, Byoung-Hee ; Yun, Hanool ; Jeong, Hee-Jin</creator><creatorcontrib>Park, Byoung-Hee ; Yun, Hanool ; Jeong, Hee-Jin</creatorcontrib><description>Hemophilia B is a congenital bleeding disorder caused by factor IX (FIX) deficiency. Generation of recombinant FIX (rFIX) is required for detecting a Hemophilia B indicator, anti-FIX antibody. In this study, we described a method for producing recombinant FIX (rFIX) using Escherichia coli. We constructed a FIX-expressing plasmid without a fusion tag protein-encoding gene and produced rFIX as a soluble form within five days. Dose-dependent curve was obtained from ELISA using anti-FIX antibody, indicating that the rFIX can be used as an antigen to detect anti-FIX antibody with high affinity and sensitivity. •Generation of a recombinant factor IX (rFIX) to compensate for detecting anti-FIX antibody in patients with hemophilia B.•Expression of a soluble form of full-length rFIX with no fusion sequence.•Confirmation of the binding efficiency of rFIX to anti-FIX antibody.</description><identifier>ISSN: 2405-5808</identifier><identifier>EISSN: 2405-5808</identifier><identifier>DOI: 10.1016/j.bbrep.2024.101714</identifier><identifier>PMID: 38681671</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Escherichia coli ; Factor IX ; Hemophilia ; Protein expression ; Recombinant protein</subject><ispartof>Biochemistry and biophysics reports, 2024-07, Vol.38, p.101714-101714, Article 101714</ispartof><rights>2024 The Authors</rights><rights>2024 The Authors.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c420t-fbd620ae6e8ff1e066e18d1285aa1c74d8fe637c372c3f6f600b2c176b7824363</cites><orcidid>0000-0001-5897-1756</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S2405580824000785$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38681671$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Byoung-Hee</creatorcontrib><creatorcontrib>Yun, Hanool</creatorcontrib><creatorcontrib>Jeong, Hee-Jin</creatorcontrib><title>Soluble expression of recombinant coagulation factor IX protein using Escherichia coli</title><title>Biochemistry and biophysics reports</title><addtitle>Biochem Biophys Rep</addtitle><description>Hemophilia B is a congenital bleeding disorder caused by factor IX (FIX) deficiency. Generation of recombinant FIX (rFIX) is required for detecting a Hemophilia B indicator, anti-FIX antibody. In this study, we described a method for producing recombinant FIX (rFIX) using Escherichia coli. We constructed a FIX-expressing plasmid without a fusion tag protein-encoding gene and produced rFIX as a soluble form within five days. Dose-dependent curve was obtained from ELISA using anti-FIX antibody, indicating that the rFIX can be used as an antigen to detect anti-FIX antibody with high affinity and sensitivity. •Generation of a recombinant factor IX (rFIX) to compensate for detecting anti-FIX antibody in patients with hemophilia B.•Expression of a soluble form of full-length rFIX with no fusion sequence.•Confirmation of the binding efficiency of rFIX to anti-FIX antibody.</description><subject>Escherichia coli</subject><subject>Factor IX</subject><subject>Hemophilia</subject><subject>Protein expression</subject><subject>Recombinant protein</subject><issn>2405-5808</issn><issn>2405-5808</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9kU1PGzEQhq2KChDlFyBVe-SSMP5Y2zn0gBAtkZB66Id6s2zvODjarFN7F9F_X4elqCdOtsbvvO-MH0IuKCwpUHm1XTqXcb9kwMShoqh4R06ZgHbRatBH_91PyHkpWwCgLdMtk8fkhGupqVT0lPz8lvrJ9djg0z5jKTENTQpNRp92Lg52GBuf7Gbq7Xh4CtaPKTfrX80-pxHj0EwlDpvmtvgHzNE_RFv1ffxA3gfbFzx_Oc_Ij8-332_uFvdfv6xvru8XXjAYF8F1koFFiToEiiAlUt3ROqa11CvR6YCSK88V8zzIIAEc81RJpzQTXPIzsp59u2S3Zp_jzuY_Jtlongspb4zNY_Q9mtYp4aztmJZKWKAuKEW5W_mVCK1vXfW6nL3qar8nLKPZxeKx7-2AaSqGg9BiJVoKVcpnqc-plIzhNZqCOfAxW_PMxxz4mJlP7fr4EjC5HXavPf9oVMGnWYD1yx4jZlN8xMFjFyuQse4U3wz4CzoaocQ</recordid><startdate>202407</startdate><enddate>202407</enddate><creator>Park, Byoung-Hee</creator><creator>Yun, Hanool</creator><creator>Jeong, Hee-Jin</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5897-1756</orcidid></search><sort><creationdate>202407</creationdate><title>Soluble expression of recombinant coagulation factor IX protein using Escherichia coli</title><author>Park, Byoung-Hee ; Yun, Hanool ; Jeong, Hee-Jin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-fbd620ae6e8ff1e066e18d1285aa1c74d8fe637c372c3f6f600b2c176b7824363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Escherichia coli</topic><topic>Factor IX</topic><topic>Hemophilia</topic><topic>Protein expression</topic><topic>Recombinant protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Byoung-Hee</creatorcontrib><creatorcontrib>Yun, Hanool</creatorcontrib><creatorcontrib>Jeong, Hee-Jin</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Directory of Open Access Journals</collection><jtitle>Biochemistry and biophysics reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Byoung-Hee</au><au>Yun, Hanool</au><au>Jeong, Hee-Jin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Soluble expression of recombinant coagulation factor IX protein using Escherichia coli</atitle><jtitle>Biochemistry and biophysics reports</jtitle><addtitle>Biochem Biophys Rep</addtitle><date>2024-07</date><risdate>2024</risdate><volume>38</volume><spage>101714</spage><epage>101714</epage><pages>101714-101714</pages><artnum>101714</artnum><issn>2405-5808</issn><eissn>2405-5808</eissn><abstract>Hemophilia B is a congenital bleeding disorder caused by factor IX (FIX) deficiency. Generation of recombinant FIX (rFIX) is required for detecting a Hemophilia B indicator, anti-FIX antibody. In this study, we described a method for producing recombinant FIX (rFIX) using Escherichia coli. We constructed a FIX-expressing plasmid without a fusion tag protein-encoding gene and produced rFIX as a soluble form within five days. Dose-dependent curve was obtained from ELISA using anti-FIX antibody, indicating that the rFIX can be used as an antigen to detect anti-FIX antibody with high affinity and sensitivity. •Generation of a recombinant factor IX (rFIX) to compensate for detecting anti-FIX antibody in patients with hemophilia B.•Expression of a soluble form of full-length rFIX with no fusion sequence.•Confirmation of the binding efficiency of rFIX to anti-FIX antibody.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>38681671</pmid><doi>10.1016/j.bbrep.2024.101714</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-5897-1756</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2405-5808
ispartof Biochemistry and biophysics reports, 2024-07, Vol.38, p.101714-101714, Article 101714
issn 2405-5808
2405-5808
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_5b74baad28674a01bf7713b9c94f5c5b
source PubMed Central Free; ScienceDirect®
subjects Escherichia coli
Factor IX
Hemophilia
Protein expression
Recombinant protein
title Soluble expression of recombinant coagulation factor IX protein using Escherichia coli
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T22%3A41%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Soluble%20expression%20of%20recombinant%20coagulation%20factor%20IX%20protein%20using%20Escherichia%20coli&rft.jtitle=Biochemistry%20and%20biophysics%20reports&rft.au=Park,%20Byoung-Hee&rft.date=2024-07&rft.volume=38&rft.spage=101714&rft.epage=101714&rft.pages=101714-101714&rft.artnum=101714&rft.issn=2405-5808&rft.eissn=2405-5808&rft_id=info:doi/10.1016/j.bbrep.2024.101714&rft_dat=%3Cproquest_doaj_%3E3048494510%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c420t-fbd620ae6e8ff1e066e18d1285aa1c74d8fe637c372c3f6f600b2c176b7824363%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3048494510&rft_id=info:pmid/38681671&rfr_iscdi=true