Prevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska

We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT- SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen...

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Bibliographic Details
Published in:Emerging infectious diseases 2000-09, Vol.6 (5), p.530-533
Main Authors: Fey, P D, Wickert, R S, Rupp, M E, Safranek, T J, Hinrichs, S H
Format: Article
Language:English
Subjects:
0157:H7
Cultures (Biology)
Diarrhea
Diarrhea - epidemiology
Diarrhea - microbiology
E. Coli
Escherichia Coli
Escherichia coli O157 - classification
Escherichia coli O157 - metabolism
Escherichia coli O157 - pathogenicity
Feces
Feces - microbiology
Humans
Immunoassay
Immunoenzyme Techniques
Nebraska
Nebraska - epidemiology
Polymerase Chain Reaction
Prevalence
Serotyping - methods
Shiga Toxin - biosynthesis
Shiga Toxin - isolation & purification
United States
USA, Nebraska
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Summary:We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT- SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx1,2 PCR [14 of 14]). Six contained serogroup O157, while non-O157 were as prevalent as O157 serogroups.
ISSN:1080-6040
1080-6059
DOI:10.3201/eid0605.000513