Genomic Characterization of β-Glucuronidase-Positive Escherichia coli O157:H7 Producing Stx2a

Among Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a β-glucuronidase-positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mi...

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Bibliographic Details
Published in:Emerging infectious diseases 2018-12, Vol.24 (12), p.2219-2227
Main Authors: Ogura, Yoshitoshi, Seto, Kazuko, Morimoto, Yo, Nakamura, Keiji, Sato, Mitsuhiko P, Gotoh, Yasuhiro, Itoh, Takehiko, Toyoda, Atsushi, Ohnishi, Makoto, Hayashi, Tetsuya
Format: Article
Language:English
Subjects:
bacteria
Computational Biology - methods
DNA Transposable Elements
Escherichia coli
Escherichia coli Infections - microbiology
Escherichia coli O157 - classification
Escherichia coli O157 - drug effects
Escherichia coli O157 - genetics
Escherichia coli O157 - metabolism
genome
Genome, Bacterial
Genomic Characterization of β-Glucuronidase–Positive
Genomics - methods
Glucuronidase - metabolism
GP STEC O157:H7
Mitomycin - pharmacology
Phylogeny
Polymorphism, Single Nucleotide
Shiga toxin
Shiga Toxin 2 - biosynthesis
Stx2a
Type III Secretion Systems - genetics
Type III Secretion Systems - metabolism
Virulence - genetics
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Summary:Among Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a β-glucuronidase-positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mild infections; Stx2a-producing strains have not been reported. We isolated, from a patient with bloody diarrhea, a GP STEC O157:H7 strain (PV15-279) that produces Stx2a in addition to Stx1a and Stx2c. Genomic comparison with other STEC O157 strains revealed that PV15-279 recently emerged from the stx1a/stx2c-positive GP STEC O157:H7 clone circulating in Japan. Major virulence genes are shared between typical (β-glucuronidase-negative) and GP STEC O157:H7 strains, and the Stx2-producing ability of PV15-279 is comparable to that of typical STEC O157:H7 strains; therefore, PV15-279 presents a virulence potential similar to that of typical STEC O157:H7. This study reveals the importance of GP O157:H7 as a source of highly pathogenic STEC clones.
ISSN:1080-6040
1080-6059
DOI:10.3201/eid2412.180404