ROMO1 is required for mitochondrial metabolism during preimplantation embryo development in pigs

Reactive oxygen species (ROS) modulator 1 (ROMO1) is a mitochondrial membrane protein that is essential for the regulation of mitochondrial ROS production and redox sensing. ROMO1 regulates ROS generation within cells and is involved in cellular processes, such as cell proliferation, senescence, and...

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Bibliographic Details
Published in:Cell division 2021-12, Vol.16 (1), p.7-7, Article 7
Main Authors: Zhou, Dongjie, Sun, Ming-Hong, Lee, Song-Hee, Cui, Xiang-Shun
Format: Article
Language:English
Subjects:
Adenosine triphosphate
Apoptosis
Cell death
Cell growth
Cell proliferation
Cytochrome
Cytochrome c
Embryo development
Embryogenesis
Embryos
Hogs
In vitro fertilization
Isoforms
Membrane potential
Membrane proteins
Metabolism
Microscopy
Mitochondria
Mitochondrial DNA
Morphology
Phosphorylation
Porcine
Proteins
Reactive oxygen species
ROMO1
Senescence
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Summary:Reactive oxygen species (ROS) modulator 1 (ROMO1) is a mitochondrial membrane protein that is essential for the regulation of mitochondrial ROS production and redox sensing. ROMO1 regulates ROS generation within cells and is involved in cellular processes, such as cell proliferation, senescence, and death. Our purpose is to investigates the impact of ROMO1 on the mitochondria during porcine embryogenesis. We found that high expression of ROMO1 was associated with porcine preimplantation embryo development, indicating that ROMO1 may contribute to the progression of embryogenesis. Knockdown of ROMO1 disrupted porcine embryo development and blastocyst quality, thereby inducing ROS production and decreasing mitochondrial membrane potential. Knockdown of ROMO1 induced mitochondrial dysfunction by disrupting the balance of OPA1 isoforms to release cytochrome c, reduce ATP, and induce apoptosis. Meanwhile, ROMO1 overexpression showed similar effects as ROMO1 KD on the embryos. Overexpression of ROMO1 rescued the ROMO1 KD-induced defects in embryo development, mitochondrial fragmentation, and apoptosis. ROMO1 plays a critical role in embryo development by regulating mitochondrial morphology, function, and apoptosis in pigs.
ISSN:1747-1028
1747-1028
DOI:10.1186/s13008-021-00076-7