Strategies for Improving Small-Molecule Biosensors in Bacteria

In recent years, small-molecule biosensors have become increasingly important in synthetic biology and biochemistry, with numerous new applications continuing to be developed throughout the field. For many biosensors, however, their utility is hindered by poor functionality. Here, we review the know...

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Bibliographic Details
Published in:Biosensors (Basel) 2022-01, Vol.12 (2), p.64
Main Authors: Miller, Corwin A, Ho, Joanne M L, Bennett, Matthew R
Format: Article
Language:English
Subjects:
Bacteria
bacterial biosensor
Binding sites
biosensor engineering
Biosensors
Gene expression
genetic circuits
genetic engineering
Laboratories
Ligands
protein engineering
Proteins
Review
Sensors
Transcription factors
whole-cell biosensor
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Summary:In recent years, small-molecule biosensors have become increasingly important in synthetic biology and biochemistry, with numerous new applications continuing to be developed throughout the field. For many biosensors, however, their utility is hindered by poor functionality. Here, we review the known types of mechanisms of biosensors within bacterial cells, and the types of approaches for optimizing different biosensor functional parameters. Discussed approaches for improving biosensor functionality include methods of directly engineering biosensor genes, considerations for choosing genetic reporters, approaches for tuning gene expression, and strategies for incorporating additional genetic modules.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12020064