circ_0008285 Regulates Glioma Progression via the miR-384/HMGB1 Axis

Background. Recent studies indicate that circular RNAs (circRNAs) have been implicated in the initiation or progression of a wide spectrum of diseases. In the current study, we explored the potential engagement of circ_0008285 in glioma and investigated the downstream regulators. Methods. The detect...

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Bibliographic Details
Published in:International journal of genomics 2023-08, Vol.2023, p.1680634-14
Main Authors: Yan, Manli, Hu, Caihong, Hu, Qi, Ma, Heran, Lei, Changjiang, Liu, Yamei
Format: Article
Language:English
Subjects:
Apoptosis
Brain cancer
Cell cycle
Cell proliferation
Circular RNA
Flow cytometry
Glioma
Glioma cells
High mobility group proteins
HMGB1 protein
Medical prognosis
MicroRNAs
Phenotypes
Polymerase chain reaction
Proteins
Radiation therapy
Reagents
Tumorigenesis
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Summary:Background. Recent studies indicate that circular RNAs (circRNAs) have been implicated in the initiation or progression of a wide spectrum of diseases. In the current study, we explored the potential engagement of circ_0008285 in glioma and investigated the downstream regulators. Methods. The detection of circ_0008285 level in glioma specimens and cell lines was conducted by quantitative real-time polymerase chain reaction. The chi-squared test was employed to evaluate the relationship between the circ_0008285 level and the clinical features of glioma patients. The roles of circ_0008285 on the proliferation and apoptosis of glioma cells were studied by knockdown experiment. Meanwhile, the regulatory relationship of circ_0008285, miR-384, and high mobility group protein B1 (HMGB1) was explored in glioma cells, and we explored the effects of circ_0008285/miR-384/HMGB1 pathway on glioma cells. Results. In glioma specimens and cell lines, the expression of circ_0008285 was significantly increased, and a high circ_0008285 level was associated with a larger tumor size and more advanced grading in glioma patients. Furthermore, downregulating circ_0008285 suppressed proliferation and triggered apoptosis of glioma cells, which was associated with a cell cycle arrest at the G1/G0 phase. Mechanism studies indicated that circ_0008285 regulated HMGB1 by sponging miR-384. Functional experiments demonstrated that circ_0008285 promoted the malignant phenotype of glioma cells by miR-384/HMGB1 axis. Conclusion. Our study revealed circ_0008285 as a novel oncogenic factor in glioma through modulating the miR-384/HMGB1 pathway, suggesting that targeting circ_0008285 could serve as a strategy for glioma management.
ISSN:2314-436X
2314-4378
DOI:10.1155/2023/1680634